Shewanella intestini sp nov., isolated from the intestine of abalone, Haliotis diversicolor

A Gram-stain-negative, rod-shaped bacterium with a singular polar flagellum, designated as strain XMDDZSB0408 T, was isolated from the intestine of adult abalone, Haliotis diversicolor. Activity of oxidase was positive and catalase activity was negative. It could grow at salinities from 3 to 6% NaCl (w/v), and pH 7-9. It had the highest sequence similarity of < 96.0% with all previously established species for the complete 16S rRNA gene (1531 bp). The results of phylogenetic analysis indicated that the strain was affiliated to the genus Shewanella and closely related to Shewanella gaetbuli TF-27(T) and Shewanella japonica KMM 3299(T) (95.8% sequence similarity), Shewanella electrodiphila MAR441(T) (95.6 %), Shewanella pacifica KMM 3597(T) (95.4 %), Shewanella donghaensis LT17(T) (95.3 %) and Shewanella olleyana ACEM 9(T) (94.7 %). The respiratory quinones were MK-7, Q-8, Q-7, MK-8, Q-7 (H4) and Q-6. The predominant fatty acids consisted of C-16: 0, summed feature 3 (comprised of C-16 : 1 omega 7c/C-16 : 1 omega 6c), C-18 : 0, summed feature 8 (comprised of C-18 : 1 omega 7c/C-18 : 1 omega 6c), C-12 : 0 and C-14 : 0. The polar lipids were identified as phosphatidylethanolamine (PE), a glycolipid (GL), a phospholipid (PL) and one unidentified lipid (L). The DNA G+C content was 41.4 mol% calculated from the draft genome sequence. On the basis of its polyphasic taxonomic properties, strain XMDDZSB0408(T) represented a novel species, for which the name Shewanella intestini sp. nov. was proposed, with the type strain XMDDZSB0408(T) (= KCTC 52125(T) = MCCC 1A01895(T)).