Primary structure of Neurospora crassa gamma-tubulin

被引：1

作者：

Heckmann, S ^{[1
]}

Schliwa, M ^{[1
]}

KubeGranderath, E ^{[1
]}

机构：

[1] ADOLF BUTENANDT INST CELL BIOL,D-80336 MUNICH,GERMANY

来源：

GENE | 1997年 / 199卷 / 1-2期

关键词：

cDNA cloning; tubulin protein family; phylogenetic tree; nucleotide sequence;

D O I：

10.1016/S0378-1119(97)00410-1

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

We report the isolation and characterization of a cDNA-clone for gamma-tubulin from N. crassa. The deduced amino-acid sequence of the cDNA-clone most resembles that of gamma-tubulins from other fungi. Differential Southern blot analysis demonstrates that most probably a single gene represents gamma-tubulin. In addition, Northern blot analysis shows that most probably only one single mRNA encoding gamma-tubulin is transcribed in N. crassa. In a phylogenetic tree that includes all known gamma-tubulins, N. crassa gamma-tubulin is grouped, as expected, together with other fungal gamma-tubulins. (C) 1997 Elsevier Science B.V.

引用

页码：303 / 309

页数：7

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