Water supply offered by alternative substrates used in in vitro Pinus taeda L. germination

被引:0
|
作者
Golle, Diego Pascoal [1 ,2 ]
Silveira Reiniger, Lia Rejane [2 ]
Briao Muniz, Marlove Fatima [3 ]
Curti, Aline Ritter [2 ]
da Rosa, Felippe Correa [2 ]
机构
[1] Univ Fed Santa Maria, CCR, Programa Posgrad Engn Florestal, BR-97119900 Santa Maria, RS, Brazil
[2] Univ Fed Santa Maria, CCR, Dept Fitotecnia, BR-97105900 Santa Maria, RS, Brazil
[3] Univ Fed Santa Maria, CCR, Dept Defesa Fitossanit, BR-97105900 Santa Maria, RS, Brazil
来源
CIENCIA RURAL | 2009年 / 39卷 / 07期
关键词
forestry seeds; tissue culture; contamination; SOMATIC EMBRYOGENESIS; REGENERATION;
D O I
10.1590/S0103-84782009005000156
中图分类号
S3 [农学(农艺学)];
学科分类号
0901 ;
摘要
The aim of this study was to assess the water supply provided by different alternative substrates as well as to analyze its efficiency in the sanity and in the in vitro germination of Pinus taeda L. seeds. The seeds were inoculated in filter paper, hydrophile cotton and agar-water medium. Water absorption by seeds was assessed for 3, 6, 9, 24, 48 and 72h and, later, at 21 days of cultivation and contamination by microorganisms was determined. Water absorption, based on the percentage increase which occurred in the weight of seeds, was higher in filter paper and hydrophile cotton. The best germination rates were obtained with filter paper and agar-water. However, agar-water presented the highest contamination rates. Due to water loss in the substrate filter-paper during cultivation, it is suggested the use of cotton because of the low contamination rates observed.
引用
收藏
页码:2218 / 2221
页数:4
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