Crystallization and preliminary X-ray study of chloroplast glyceraldehyde-3-phosphate dehydrogenase

被引：6
|
作者：
Sabatino, P
Fermani, S
Ripamonti, A
Cassetta, A
Scagliarini, S
Trost, P
机构：
[1] Univ Bologna, Dipartimento Chim G Ciamician, I-40126 Bologna, Italy
[2] CNR, Ist Strutturist Chim, Trieste, Italy
[3] Univ Bologna, Dipartimento Biol Evoluzionist Sperimentale, I-40126 Bologna, Italy
来源：
ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY | 1999年 / 55卷
关键词：
D O I：
10.1107/S090744499801302X
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Glyceraldehyde-3-phosphate dehydrogenase from spinach chloroplasts has been crystallized by vapour diffusion in the pH range 7-8.5 in (NH4)(2)SO4 and Tris-HCl buffer or potassium phosphate buffer at room temperature. Crystals of the A(4) isoform, grown at pH 8.5 in Tris-HCl buffer, diffract to 3.0 Angstrom (at 100 K) using synchrotron radiation. The crystals belong to the orthorhombic C222 space group, with unit-cell dimensions a = 145.9, b = 185.9 and c = 106.3 Angstrom, and probably contain one tetramer per asymmetric unit. Structure determination by molecular replacement is in progress.
引用
收藏
页码：566 / 567
页数：2
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