Early embryonic development and transplantation in tree shrews

被引:6
|
作者
Yan, Lan-Zhen [1 ]
Sun, Bin [2 ]
Lyu, Long-Bao [1 ]
Ma, Yu-Hua [1 ]
Chen, Jia-Qi [1 ]
Lin, Qing [3 ]
Zheng, Ping [1 ,4 ]
Zhao, Xu-Dong [1 ,2 ]
机构
[1] Chinese Acad Sci, Kunming Inst Zool, Kunming Primate Res Ctr, Kunming 650223, Yunnan, Peoples R China
[2] Chinese Acad Sci, Kunming Inst Zool, Chinese Acad Sci & Yunnan Prov, Key Lab Anim Models & Human Dis Mech, Kunming 650223, Yunnan, Peoples R China
[3] Yunnan Univ Tradit Chinese Med, Kunming 650500, Yunnan, Peoples R China
[4] Chinese Acad Sci, Kunming Inst Zool, State Key Lab Genet Resources & Evolut, Kunming 650223, Yunnan, Peoples R China
关键词
Embryo culture; Embryonic developmental stage; Tree shrew;
D O I
10.13918/j.issn.2095-8137.2016.4.252
中图分类号
Q95 [动物学];
学科分类号
071002 ;
摘要
As a novel experimental animal model, tree shrews have received increasing attention in recent years. Despite this, little is known in regards to the time phases of their embryonic development. In this study, surveillance systems were used to record the behavior and timing of copulations; embryos at different post-copulation stages were collected and cultured in vitro; and the developmental characteristics of both early-stage and in vitro cultured embryos were determined. A total of 163 females were collected following effective copulation, and 150 were used in either unilateral or bilateral oviduct embryo collections, with 307 embryos from 111 females obtained (conception rate=74%). Among them, 237 embryos were collected from 78 females, bilaterally, i.e., the average embryo number per female was 3.04; 172 fertilized eggs collected from 55 females, bilaterally, were cultured for 24-108 h in vitro for developmental observations; finally, 65 embryos from 23 bilateral cases and 70 embryos from 33 unilateral cases were used in embryo transplantation.
引用
收藏
页码:252 / 258
页数:7
相关论文
共 50 条
  • [1] Early embryonic development and transplantation in tree shrews
    Lan-Zhen YAN
    Bin SUN
    Long-Bao LYU
    Yu-Hua MA
    Jia-Qi CHEN
    Qing LIN
    Ping ZHENG
    Xu-Dong ZHAO
    [J]. Zoological Research, 2016, 37 (04) : 252 - 258
  • [2] Depletion of endogenous germ cells in tree shrews in preparation for spermatogonial transplantation
    Liu, Tingting
    Guo, Ying
    Yan, Lanzhen
    Sun, Bin
    Zheng, Ping
    Zhao, Xudong
    [J]. EXPERIMENTAL AND THERAPEUTIC MEDICINE, 2017, 14 (03) : 2349 - 2354
  • [3] Heterogenous thinning of peripapillary tissues occurs early during high myopia development in juvenile tree shrews
    Khalafallah, Mahmoud T.
    Fuchs, Preston A.
    Nugen, Fred
    El Hamdaoui, Mustapha
    Levy, Alexander M.
    Samuels, Brian C.
    Grytz, Rafael
    [J]. EXPERIMENTAL EYE RESEARCH, 2024, 240
  • [4] REPRODUCTION IN TREE SHREWS
    CONAWAY, CH
    SORENSON, MW
    [J]. JOURNAL OF REPRODUCTION AND FERTILITY, 1965, 9 (03): : 389 - &
  • [5] Using Tree Shrews (Tupaia belangeri) as a Novel Animal Model of Liver Transplantation
    Bo Tang
    Tao Wu
    Shu-feng Xiao
    Jia-yun Ge
    Dong Wei
    Chun-man Li
    Qiu-hong Wang
    Wang Zeng
    Bi-mang Fu
    Jie Zhang
    [J]. Current Medical Science, 2018, 38 : 1069 - 1074
  • [6] Using Tree Shrews (Tupaia belangeri) as a Novel Animal Model of Liver Transplantation
    Tang, Bo
    Wu, Tao
    Xiao, Shu-feng
    Ge, Jia-yun
    Wei, Dong
    Li, Chun-man
    Wang, Qiu-hong
    Zeng, Wang
    Fu, Bi-mang
    Zhang, Jie
    [J]. CURRENT MEDICAL SCIENCE, 2018, 38 (06) : 1069 - 1074
  • [7] EXPERIMENTAL MYOPIA IN TREE SHREWS
    NORTON, TT
    [J]. CIBA FOUNDATION SYMPOSIA, 1990, 155 : 178 - 199
  • [8] TAXONOMIC STATUS OF TREE SHREWS
    CAMPBELL, CB
    [J]. SCIENCE, 1966, 153 (3734) : 436 - &
  • [9] PHYLETIC POSITION OF TREE SHREWS
    GOODMAN, M
    [J]. SCIENCE, 1966, 153 (3743) : 1550 - &
  • [10] THE DEVELOPMENT OF VISUAL-ACUITY IN TREE SHREWS, BUSHBABIES AND SLOW LORIS
    HERTENSTEIN, B
    ZIMMERMANN, E
    RAHMANN, H
    [J]. INTERNATIONAL JOURNAL OF PRIMATOLOGY, 1987, 8 (05) : 533 - 533