Understanding the transactions that occur between humans and their environments requires research focused on phenomena that explain behavioral patterns, particularly values that serve as guiding principles in life. Mounting evidence has suggested that pro-environmental behavior is motivated by the long-term goal of living a meaningful life, as reflected by Aristotle's concept of eudaimonia. However, the relationships among value concepts, particularly eudaimonic values, remains unclear despite the fundamental role that these constructs play in explaining why people make behavioral decisions. We conceptualized eudaimonic values with guidance from Self-Determination Theory to understand how a suite of values affected pro-environmental behavior reported by recreational anglers (n = 1,103) across five US states (Wisconsin, Illinois, Michigan, New York, Indiana) in the Great Lakes region. Results from a latent variable path model showed that eudaimonic values were strong predictors of biospheric, altruistic, egoistic, and hedonic values, which in turn, influenced self-reported behavior among recreational anglers who were at risk of spreading aquatic invasive species. These findings suggest that eudaimonic values are an antecedent to values-behavior relationships and can improve the predictive capacity of models being developed to inform management strategies for minimizing human activities that are contributing to the unintentional spread of aquatic invasive species.