Curcumin Loaded Eudragit S100/PLGA Nanoparticles in Treatment of Colon Cancer: Formulation, Optimization, and in-vitro Cytotoxicity Study

被引:4
|
作者
Saraf, Apeksha [1 ]
Dubey, Nidhi [1 ]
Dubey, Nitin [2 ]
Sharma, Mayank [3 ]
机构
[1] Devi Ahilya Vishwavidyalaya, Sch Pharm, Takshashila Campus,Khandwa Rd, Indore 452001, Madhya Pradesh, India
[2] IPS Acad, Coll Pharm, AB Rd, Indore, Madhya Pradesh, India
[3] SVKMs NMIMS, Sch Pharm & Technol Management, Dhule, Maharashtra, India
关键词
Colon cancer; Curcumin; Nanoparticles; Cytotoxicity; Nanoprecipitation; Box-Behnken; BOX-BEHNKEN DESIGN; PH-SENSITIVE NANOPARTICLES; DRUG-DELIVERY; COLORECTAL-CANCER; POLYMERIC NANOPARTICLES; ANTICANCER ACTIVITY; SYSTEMS; BIOAVAILABILITY; STABILITY; APOPTOSIS;
D O I
10.5530/ijper.55.2s.114
中图分类号
G40 [教育学];
学科分类号
040101 ; 120403 ;
摘要
Introduction: Polymeric nanoparticles provide a promising strategy for site-specific delivery of dietary phytochemicals in the treatment of colon cancer. Curcumin (CU) is a dietary phytochemical with well-proven anti-cancer activity in colon cancer. However, its clinical application is confined because of its hydrophobicity, lack of selectivity towards tumor tissues, and poor bioavailability. Objectives: The main objective of the study was to enhance the selectivity and cytotoxicity of poorly water-soluble curcumin by fabricating polymeric nanoparticles (NPs). Methods: In the present investigation, localized delivery of CU to the colon was achieved by employing a combination of Eudragit S100 (ES100), as a pH-sensitive polymer and polylactic-co-glycolic acid (PLGA), as a biodegradable polymer. The curcumin loaded dual-functional NPs were prepared by nanoprecipitation method and optimized using Box-Behnken experimental design. Results: The in- vitro cytotoxicity study of Curcumin Nanoparticles (CU-NPs) in CT26 murine colon carcinoma cells showed higher cytotoxicity in comparison to free drugs. The IC50 values of free curcumin and NPs containing curcumin was found to be 1.43 +/- 0.08 mu g/ml and 0.25 +/- 0.12 mu g/ml, respectively ( p <0.05). Conclusion: The results of the study suggested that the dual functional polymeric NPs exhibited a remarkably promising carrier system for effective delivery of such poorly water-soluble dietary phytochemicals in colon cancer.
引用
收藏
页码:S428 / S440
页数:13
相关论文
共 50 条
  • [1] Enhancement of cytotoxicty of diallyl disulfide toward colon cancer by Eudragit S100/PLGA nanoparticles
    Saraf, Apeksha
    Dubey, Nidhi
    Dubey, Nitin
    Sharma, Mayank
    [J]. JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY, 2021, 64
  • [2] Factorial design formulation optimization and in vitro characterization of curcumin-loaded PLGA nanoparticles for colon delivery
    Akl, Mohamed A.
    Kartal-Hodzic, Alma
    Oksanen, Timo
    Ismael, Hatem R.
    Afouna, Mohsen M.
    Yliperttula, Marjo
    Samy, Ahmed M.
    Viitala, Tapani
    [J]. JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY, 2016, 32 : 10 - 20
  • [3] Formulation and In Vitro - In Vivo Evaluation of Quercetin Loaded Eudragit S100 Microspheres
    Jat, Ramchandra
    Jain, Suman
    Singh, S. K.
    Gupta, Rishikesh
    [J]. ASIAN JOURNAL OF PHARMACEUTICS, 2018, 12 (01) : 31 - 37
  • [4] Formulation of Cabotegravir Loaded Gold Nanoparticles: Optimization, Characterization to In-Vitro Cytotoxicity Study
    Rawat, Purnima
    Imam, Syed Sarim
    Gupta, Sharad
    [J]. JOURNAL OF CLUSTER SCIENCE, 2023, 34 (02) : 893 - 905
  • [5] Formulation of Cabotegravir Loaded Gold Nanoparticles: Optimization, Characterization to In-Vitro Cytotoxicity Study
    Purnima Rawat
    Syed Sarim Imam
    Sharad Gupta
    [J]. Journal of Cluster Science, 2023, 34 : 893 - 905
  • [6] Eudragit®-S100 Coated PLGA Nanoparticles for Colon Targeting of Etoricoxib: Optimization and Pharmacokinetic Assessments in Healthy Human Volunteers
    El-Maghawry, Enas
    Tadros, Mina, I
    Elkheshen, Seham A.
    Abd-Elbary, Ahmed
    [J]. INTERNATIONAL JOURNAL OF NANOMEDICINE, 2020, 15 : 3965 - 3980
  • [7] Formulation and optimization of celecoxib-loaded PLGA nanoparticles by the Taguchi design and their in vitro cytotoxicity for lung cancer therapy
    Emami, Jaber
    Pourmashhadi, Aida
    Sadeghi, Hojat
    Varshosaz, Jaleh
    Hamishehkar, Hamed
    [J]. PHARMACEUTICAL DEVELOPMENT AND TECHNOLOGY, 2015, 20 (07) : 791 - 800
  • [8] Eudragit® S100 coated calcium pectinate microspheres of curcumin for colon targeting
    Zhang, Lin
    Cao, Fengliang
    Ding, Buyun
    Li, Qilu
    Xi, Yanwei
    Zhai, Guangxi
    [J]. JOURNAL OF MICROENCAPSULATION, 2011, 28 (07) : 659 - 667
  • [9] Formulation optimization of double emulsification method for preparation of enzyme-loaded Eudragit S100 microspheres
    Rawat, Manju
    Saraf, Swarnlata
    [J]. JOURNAL OF MICROENCAPSULATION, 2009, 26 (04) : 306 - 314
  • [10] Formulation and In vitro Characterization of Eudragit® L100 and Eudragit® L100-PLGA Nanoparticles Containing Diclofenac Sodium
    Cetin, Meltem
    Atila, Alptug
    Kadioglu, Yucel
    [J]. AAPS PHARMSCITECH, 2010, 11 (03): : 1250 - 1256