The correlation between the benign and malignant lesions of colorectal tumors and the CYP24A1 gene polymorphism

Objective: To explore the correlation between the CYP24A1 gene polymorphism and colorectal tumor patients' benign and malignant lesions and analyze the relationship between the CYP24A1 gene and colorectal cancer susceptibility. Methods: A total of 257 colorectal tumor patients diagnosed for the first time and admitted to our hospital were recruited as the study cohort and divided into the malignant group (n=139) and the benign group (n=118) according to their pathological examination results. A total of 110 healthy patients who visited our hospital during the same period for physical exams were recruited as the control group. The individual clinical data and blood samples from the patients in each group were collected and recorded. The gene fragments at the four loci (rs114368325, rs6068812, rs2296239, and rs1570670) in the CYP24A1 gene were detected using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP), and inter-group difference and logistic regression analyses were performed to explore the relationship between the gene polymorphism and the benign and malignant lesions in the colorectal tumors. Results: The distribution frequencies of the loci rs114368325 and rs2296239 in the individuals in the three groups conformed to the Hardy-Weinberg equilibrium law. Two genotypes, GG and AG, were found in rs114368325, three genotypes, CC, CT, and TT, were found in rs2296239, and the GG genotype was found in rs6068812 and rs1570670. The malignant group had a significantly higher proportion of G in the locus rs114368325 and a higher GG distribution frequency than the other two groups (P<0.05). There was no significant difference in different phenotypes in the other gene loci among the three groups (P>0.05). Conclusion: There is no significant correlation between the genetic polymorphisms of the four loci (rs114368325, rs6068812, rs2296239, and rs1570670) at the CYP24A1 gene and the benign and malignant lesions of colorectal tumors, and the nature of the benign and malignant lesions in colorectal tumors can be evaluated by monitoring the locus rs114368325.