LncRNA RBM5-AS1 Promotes Osteosarcoma Cell Proliferation, Migration, and Invasion

被引：5
|
作者：
Deng, Biyong [1 ]
Pan, Runsang [2 ]
Ou, Xin [1 ]
Wang, Taizhe [1 ]
Wang, Weiguo [3 ]
Nie, Yingjie [4 ]
Chen, Houping [2 ]
机构：
[1] Guizhou Prov Orthoped Hosp, Orthoped Surg Dept, Guiyang, Guizhou, Peoples R China
[2] Guiyang Maternal & Child Hlth Care Hosp, Orthoped Surg Dept, Guiyang, Guizhou, Peoples R China
[3] Guizhou Prov Orthoped Hosp, Dept Pathol, Guiyang, Guizhou, Peoples R China
[4] Guizhou Prov Peoples Hosp, Sci & Educ Div, Guiyang 550000, Guizhou, Peoples R China
来源：
BIOMED RESEARCH INTERNATIONAL | 2021年 / 2021卷
关键词：
D O I：
10.1155/2021/5271291
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Purpose. Osteosarcoma (Os) is the most frequent malignant tumor of the bone in the pediatric age group, and accumulating evidences show that lncRNAs play a key role in the development of Os. Thus, we investigated the role of RBM5-AS1 and its molecular mechanism. Methods. The expression of RBM5-AS1 in Os tissues and cell lines was detected by real-time polymerase chain reaction (QPCR). The effect of RBM5-AS1 on the proliferation of Os cells was detected using CCK8 assays and flow cytometry. The effect of RBM5-AS1 on the migration and invasion of Os cells was detected by transwell assays. And we performed QPCR and western blotting assays to investigate the relationship between RBM5-AS1 and RBM5. Finally, western blotting assays were performed to explore the mechanism of RBM5. Results. LncRNA RBM5-AS1 was overexpressed in the Os tissues and cell lines. And lncRNA RBM5-AS1 promoted Os cell proliferation, migration, and invasion in vitro and tumor growth in vivo. LncRNA RBM5-AS1 targets RBM5 in Os cells. Conclusion. To sum up, the results showed that lncRNA RBM5-AS1 promotes cell proliferation, migration, and invasion in Os.
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页数：9
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