Integrating imaging and RNA-seq improves outcome prediction in cervical cancer

被引:11
|
作者
Zhang, Jin [1 ,2 ,3 ]
Rashmi, Ramachandran [1 ]
Inkman, Matthew [1 ]
Jayachandran, Kay [1 ]
Ruiz, Fiona [1 ]
Waters, Michael R. [1 ]
Grigsby, Perry W. [1 ]
Markovina, Stephanie [1 ,3 ]
Schwarz, Julie K. [1 ,3 ,4 ]
机构
[1] Washington Univ, Sch Med, Dept Radiat Oncol, St Louis, MO USA
[2] Washington Univ, Sch Med, Inst Informat, St Louis, MO USA
[3] Washington Univ, Sch Med, Siteman Canc Ctr, St Louis, MO USA
[4] Washington Univ, Sch Med, Dept Cell Biol & Physiol, St Louis, MO 63110 USA
来源
JOURNAL OF CLINICAL INVESTIGATION | 2021年 / 131卷 / 05期
关键词
MESENCHYMAL TRANSITION; F-18; FLUORODEOXYGLUCOSE; THERAPEUTIC TARGET; BIOMARKER; SIGNATURE; PATHWAY;
D O I
10.1172/JCI139232
中图分类号
R-3 [医学研究方法]; R3 [基础医学];
学科分类号
1001 ;
摘要
Approaches using a single type of data have been applied to classify human tumors. Here we integrate imaging features and transcriptomic data using a prospectively collected tumor bank. We demonstrate that increased maximum standardized uptake value on pretreatment F-18-fluorodeoxyglucose-positron emission tomography correlates with epithelial-to-mesenchymal transition (EMT) gene expression. We derived and validated 3 major molecular groups, namely squamous epithelial, squamous mesenchymal, and adenocarcinoma, using prospectively collected institutional (n = 67) and publicly available (n = 304) data sets. Patients with tumors of the squamous mesenchymal subtype showed inferior survival outcomes compared with the other 2 molecular groups. High mesenchymal gene expression in cervical cancer cells positively correlated with the capacity to form spheroids and with resistance to radiation. CaSki organoids were radiation-resistant but sensitive to the glycolysis inhibitor, 2-DG. These experiments provide a strategy for response prediction by integrating large data sets, and highlight the potential for metabolic therapy to influence EMT phenotypes in cervical cancer.
引用
收藏
页数:7
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