Improving the Quantification of rSIV.F/HN Vectors for Cystic Fibrosis Gene Therapy Using Droplet Digital PCR

被引：0
|
作者：
Gamlen, Toby [1 ]
Viegas, Mariana F. A. [1 ]
Dean, Rebecca J. [1 ]
Conway, Catriona C. [1 ]
Miah, Kamran M. [1 ]
Gill, Deborah R. [1 ]
Hyde, Stephen C. [1 ]
机构：
[1] Univ Oxford, Gene Med, Oxford, England
来源：
MOLECULAR THERAPY | 2020年 / 28卷 / 04期
关键词：
D O I：
暂无
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
507
引用
收藏
页码：223 / 223
页数：1
相关论文
共 10 条
  • [1] Gene therapy for cystic fibrosis using adenovirus vectors
    Pavirani, A
    Schatz, C
    Mehtali, M
    [J]. M S-MEDECINE SCIENCES, 1996, 12 (01): : 25 - 33
  • [2] Quantification of Lentiviral Vectors Using Quantitative Real-Time PCR vs Digital Droplet PCR
    Dean, Rebecca J.
    [J]. MOLECULAR THERAPY, 2022, 30 (04) : 356 - 356
  • [3] Development and Validation of Droplet Digital PCR Assay for Viral Vectors Used in Cell and Gene Therapy
    Schneider, Andrew
    Woo, Ahae
    Mckee, Marian L.
    Hasson, Bradley
    [J]. MOLECULAR THERAPY, 2018, 26 (05) : 156 - 156
  • [4] Clinical Development of an Optimal F/HN Pseudotyped SIV Vector for Cystic Fibrosis Lung Gene Therapy
    Pringle, Ian A.
    Alton, Eric W.
    Connolly, Mary M.
    Chan, Mario
    Davies, Jane C.
    Davies, Lee A.
    Gea-Sorli, Sabrina
    Gill, Deborah R.
    Griesenbach, Uta
    Hasegawa, Mamoru
    Hyde, Stephen C.
    Inness, Alastair J.
    McLachan, Gerry
    Meng, Cuixiang
    Sumner-Jones, Stephanie G.
    Boyd, Chris A.
    [J]. MOLECULAR THERAPY, 2014, 22 : S141 - S141
  • [5] Standard Free Droplet Digital PCR as a New Tool for More Precise and Reliable Quality Control of Gene Therapy Vectors
    Boehme, Philip
    Stellberger, Thorsten
    Zhang, Wenli
    Doerner, Johannes
    Jing, Liu
    Schulz, Eric
    Bergmann, Thorsten
    Solanki, Manish
    Baiker, Armin
    Ehrhardt, Anja
    [J]. MOLECULAR THERAPY, 2015, 23 : S266 - S266
  • [6] Use of a Purpose-Built Impression Cytology Device for Gene Expression Quantification at the Ocular Surface Using Quantitative PCR and Droplet Digital PCR
    Ganesalingam, Kalaivarny
    Ismail, Salim
    Craig, Jennifer P.
    Sherwin, Trevor
    [J]. CORNEA, 2019, 38 (01) : 127 - 133
  • [7] DNA Based Detection and Quantification of BCR-ABL1 Gene Using Patient-Specific qPCR and Droplet Digital PCR Tests in Chronic Myeloid Leukemia
    Zizkova, Hana
    Motlova, Eliska
    Zemanova, Karla
    Klamova, Hana
    Hovorkova, Lenka
    Zuna, Jan
    Zach, Jan
    Saussele, Susanne
    Mahon, Francois-Xavier
    Polakova, Katerina Machova
    [J]. BLOOD, 2015, 126 (23)
  • [8] Improving clinical preimplantation genetic diagnosis for cystic fibrosis by duplex PCR using two polymorphic markers or one polymorphic marker in combination with the detection of the ΔF508 mutation
    Goossens, V
    Sermon, K
    Lissens, W
    De Rycke, M
    Saerens, B
    De Vos, A
    Henderix, P
    Van de Velde, H
    Platteau, P
    Van Steirteghem, A
    Devroey, P
    Liebaers, I
    [J]. MOLECULAR HUMAN REPRODUCTION, 2003, 9 (09) : 559 - 567
  • [9] Prevalence of neutralizing antibodies against adeno-associated virus (AAV) types 2, 5, and 6 in cystic fibrosis and normal populations: Implications for gene therapy using AAV vectors
    Halbert, CL
    Miller, AD
    McNamara, S
    Emerson, J
    Gibson, RL
    Ramsey, B
    Aitken, ML
    [J]. HUMAN GENE THERAPY, 2006, 17 (04) : 440 - 447
  • [10] ABSOLUTE QUANTIFICATION OF JAK2V617F AND CALR MUTANT ALLELIC BURDEN IN PATIENTS WITH PHILADELPHIA-NEGATIVE MYELOPROLIFERATIVE NEOPLASMS (MPNS) AND SPLANCHNIC VENOUS THROMBOSIS (SVT) BY USING DROPLET DIGITAL PCR (DDPCR)
    Colaizzo, D.
    Amitrano, L.
    Guardascione, M. A.
    Sgherza, N.
    Chinni, E.
    Favuzzi, G.
    Vergura, P.
    Fischietti, L.
    Cappucci, F.
    Cascavilla, N.
    Margaglione, M.
    Grandone, E.
    [J]. HAEMATOLOGICA, 2017, 102 : 60 - 61
1