Recombinant human amyloid precursor-like protein 2 (APLP2) expressed in the yeast Pichia pastoris can stimulate neurite outgrowth

被引：51
|
作者：
Cappai, R [1 ]
Mok, SS
Galatis, D
Tucker, DF
Henry, A
Beyreuther, K
Small, DH
Masters, CL
机构：
[1] Univ Melbourne, Dept Pathol, Parkville, Vic 3052, Australia
[2] Mental Hlth Res Inst, Parkville, Vic 3052, Australia
[3] Univ Heidelberg, Ctr Mol Biol, D-69120 Heidelberg, Germany
来源：
FEBS LETTERS | 1999年 / 442卷 / 01期
关键词：
amyloid precursor-like protein 2; neuritogenesis;
D O I：
10.1016/S0014-5793(98)01635-4
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The human amyloid precursor-like protein 2 (APLP2) is a member of the Alzheimer's disease amyloid precursor protein (APP) gene family. The human APLP2 ectodomain (sAPLP2) was expressed in the yeast Pichia pastoris and the recombinant sAPLP2 was purified from the culture medium in a single step by metal-chelating Sepharose chromatography. The neuritotrophic activity of APLP2 was compared to the APP isoforms sAPP695 and sAPP751 on chick sympathetic neurones. APLP2 had neurite outgrowth-promoting activity similar to that of APP isoforms. This suggests that APP and APLP2 have a similar or related role and supports the idea of a redundancy in function between the APP-gene family proteins. (C) 1999 Federation of European Biochemical Societies.
引用
收藏
页码：95 / 98
页数：4
相关论文
共 50 条
  • [1] Expression of amyloid precursor-like protein 2 (APLP2) in glioblastoma is associated with patient prognosis
    Chen, Yihong
    Wang, Hongxiang
    Tan, Cong
    Yan, Yong
    Shen, Junyan
    Huang, Qilin
    Xu, Tao
    Lin, Junting
    Chen, Juxiang
    FOLIA NEUROPATHOLOGICA, 2018, 56 (01) : 30 - 38
  • [2] Signaling via Amyloid Precursor-Like Proteins APLP1 and APLP2
    Orcholski, Mark E.
    Zhang, Qiang
    Bredesen, Dale E.
    JOURNAL OF ALZHEIMERS DISEASE, 2011, 23 (04) : 689 - 699
  • [3] Deletion of the amyloid precursor-like protein 2 (APLP2) does not affect hippocampal neuron morphology or function
    Midthune, Brea
    Tyan, Sheue-Houy
    Walsh, Jessica J.
    Sarsoza, Floyd
    Eggert, Simone
    Hof, Patrick R.
    Dickstein, Dara L.
    Koo, Edward H.
    MOLECULAR AND CELLULAR NEUROSCIENCE, 2012, 49 (04) : 448 - 455
  • [4] REGULATION OF THE MOUSE BETA-AMYLOID PRECURSOR-LIKE PROTEIN (APLP2) GENE - PROMOTER CHARACTERIZATION AND ANALYSIS OF MICE WITH TARGETED APLP2 ALLELES
    VONKOCH, SS
    REED, RR
    ZHEN, H
    SISODIA, SS
    JOURNAL OF CELLULAR BIOCHEMISTRY, 1995, : 107 - 107
  • [5] AMYLOID PRECURSOR-LIKE PROTEIN-2 (APLP2) IS A CHONDROITIN SULFATE PROTEOGLYCAN - IDENTIFICATION OF THE GLYCOSAMINOGLYCAN MODIFICATION SITE
    THINAKARAN, G
    SISODIA, SS
    NEUROBIOLOGY OF AGING, 1994, 15 : S65 - S65
  • [6] Amyloid precursor-like protein 1 (APLP1) exhibits stronger zinc-dependent neuronal adhesion than amyloid precursor protein and APLP2
    Mayer, Magnus C.
    Schauenburg, Linda
    Thompson-Steckel, Greta
    Dunsing, Valentin
    Kaden, Daniela
    Voigt, Philipp
    Schaefer, Michael
    Chiantia, Salvatore
    Kennedy, Timothy E.
    Multhaup, Gerhard
    JOURNAL OF NEUROCHEMISTRY, 2016, 137 (02) : 266 - 276
  • [7] Identification and expression of the first nonmammalian amyloid-β precursor-like protein APLP2 in the amphibian Xenopus laevis
    Collin, RWJ
    van Strien, D
    Leunissen, JAM
    Martens, GJM
    EUROPEAN JOURNAL OF BIOCHEMISTRY, 2004, 271 (10): : 1906 - 1912
  • [8] Amyloid precursor-like protein 2 (APLP2) affects the actin cytoskeleton and increases pancreatic cancer growth and metastasis
    Pandey, Poomy
    Rachagani, Satyanarayana
    Das, Srustidhar
    Seshacharyulu, Parthasarathy
    Sheinin, Yuri
    Naslavsky, Naava
    Pan, Zenggang
    Smith, Brittney L.
    Peters, Haley L.
    Radhakrishnan, Prakash
    McKenna, Nicole R.
    Giridharan, Sai Srinivas Panapakkam
    Haridas, Dhanya
    Kaur, Sukhwinder
    Hollingsworth, Michael A.
    MacDonald, Richard G.
    Meza, Jane L.
    Caplan, Steve
    Batra, Surinder K.
    Solheim, Joyce C.
    ONCOTARGET, 2015, 6 (04) : 2064 - 2075
  • [9] Accumulation of the amyloid precursor-like protein APLP2 and reduction of APLP1 in retinoic acid-differentiated human neuroblastoma cells upon curcumin-induced neurite retraction
    Adlerz, L
    Beckman, M
    Holback, S
    Tehranian, R
    Toro, VC
    Iverfeldt, K
    MOLECULAR BRAIN RESEARCH, 2003, 119 (01): : 62 - 72
  • [10] Amyloid beta-protein stimulates parallel increases in cellular levels of its precursor and amyloid precursor-like protein 2 (APLP2) in human cerebrovascular smooth muscle cells
    SaporitoIrwin, SM
    Thinakaran, G
    Ruffini, L
    Sisodia, SS
    VanNostrand, WE
    AMYLOID-INTERNATIONAL JOURNAL OF EXPERIMENTAL AND CLINICAL INVESTIGATION, 1997, 4 (01): : 54 - 60
12345