Role of GABAergic inhibition in shaping odor-evoked spatiotemporal patterns in the Drosophila antennal lobe

被引:335
|
作者
Wilson, RI [1 ]
Laurent, G
机构
[1] Harvard Univ, Sch Med, Dept Neurobiol, Boston, MA 02115 USA
[2] CALTECH, Div Biol 139 74, Pasadena, CA 92115 USA
来源
JOURNAL OF NEUROSCIENCE | 2005年 / 25卷 / 40期
关键词
olfactory; antennal lobe; GABA; GABA(B) receptor; synaptic transmission; interneurons;
D O I
10.1523/JNEUROSCI.2070-05.2005
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
Drosophila olfactory receptor neurons project to the antennal lobe, the insect analog of the mammalian olfactory bulb. GABAergic synaptic inhibition is thought to play a critical role in olfactory processing in the antennal lobe and olfactory bulb. However, the properties of GABAergic neurons and the cellular effects of GABA have not been described in Drosophila, an important model organism for olfaction research. We have used whole-cell patch-clamp recording, pharmacology, immunohistochemistry, and genetic markers to investigate how GABAergic inhibition affects olfactory processing in the Drosophila antennal lobe. We show that many axonless local neurons (LNs) in the adult antennal lobe are GABAergic. GABA hyperpolarizes antennal lobe projection neurons (PNs) via two distinct conductances, blocked by a GABA(A)- and GABA(B)-type antagonist, respectively. Whereas GABA(A) receptors shape PN odor responses during the early phase of odor responses, GABA(B) receptors mediate odor-evoked inhibition on longer time scales. The patterns of odor-evoked GABA(B)-mediated inhibition differ across glomeruli and across odors. Finally, we show that LNs display broad but diverse morphologies and odor preferences, suggesting a cellular basis for odor- and glomerulus-dependent patterns of inhibition. Together, these results are consistent with a model in which odors elicit stimulus-specific spatial patterns of GABA release, and as a result, GABAergic inhibition increases the degree of difference between the neural representations of different odors.
引用
收藏
页码:9069 / 9079
页数:11
相关论文
共 50 条
  • [1] Calcium Imaging of Odor-evoked Responses in the Drosophila Antennal Lobe
    Silbering, Ana F.
    Bell, Rati
    Galizia, C. Giovanni
    Benton, Richard
    [J]. JOVE-JOURNAL OF VISUALIZED EXPERIMENTS, 2012, (61):
  • [2] Model of cellular and network mechanisms for odor-evoked temporal patterning in the locust antennal lobe
    Bazhenov, M
    Stopfer, M
    Rabinovich, M
    Abarbanel, HDI
    Sejnowski, TJ
    Laurent, G
    [J]. NEURON, 2001, 30 (02) : 569 - 581
  • [3] In vivo chloride imaging in the antennal lobe of Drosophila: the role of inhibition in odor processing
    Fiedler, A.
    [J]. JOURNAL OF NEUROCHEMISTRY, 2009, 110 : 55 - 56
  • [4] Calcium imaging revealed no modulatory effect on odor-evoked responses of the Drosophila antennal lobe by two populations of inhibitory local interneurons
    Martin F. Strube-Bloss
    Veit Grabe
    Bill S. Hansson
    Silke Sachse
    [J]. Scientific Reports, 7
  • [5] Calcium imaging revealed no modulatory effect on odor-evoked responses of the Drosophila antennal lobe by two populations of inhibitory local interneurons
    Strube-Bloss, Martin F.
    Grabe, Veit
    Hansson, Bill S.
    Sachse, Silke
    [J]. SCIENTIFIC REPORTS, 2017, 7
  • [6] Smell-O-Vision: Functional Imaging of Odor-Evoked Activity and Neuromodulation in the Mosquito Antennal Lobe
    Wolff, G. H.
    Riffell, J. A.
    [J]. INTEGRATIVE AND COMPARATIVE BIOLOGY, 2019, 59 : E251 - E251
  • [7] Odor-evoked inhibition of olfactory sensory neurons drives olfactory perception in Drosophila
    Li-Hui Cao
    Dong Yang
    Wei Wu
    Xiankun Zeng
    Bi-Yang Jing
    Meng-Tong Li
    Shanshan Qin
    Chao Tang
    Yuhai Tu
    Dong-Gen Luo
    [J]. Nature Communications, 8
  • [8] Optical imaging of odor-evoked glomerular activity patterns in the antennal lobes of the ant Camponotus rufipes
    Galizia, CG
    Menzel, R
    Hölldobler, B
    [J]. NATURWISSENSCHAFTEN, 1999, 86 (11) : 533 - 537
  • [9] Optical Imaging of Odor-Evoked Glomerular Activity Patterns in the Antennal Lobes of the Ant Camponotus rufipes
    C. G. Galizia
    R. Menzel
    B. Hölldobler
    [J]. Naturwissenschaften, 1999, 86 : 533 - 537
  • [10] Odor reception in antenna and antennal lobe of Drosophila
    Chakraborty, Tuhin Subhra
    Siddiqi, Obaid
    [J]. FLY, 2011, 5 (01) : 14 - 17