The creation of sugar beet transgenic plants expressing bar gene

被引：7
|
作者：
Mishutkina, Ya. V. [1 ]
Kamionskaya, A. M. [1 ]
Skryabin, K. G. [1 ]
机构：
[1] Russian Acad Sci, Bioingn Ctr, Moscow 117312, Russia
来源：
APPLIED BIOCHEMISTRY AND MICROBIOLOGY | 2010年 / 46卷 / 01期
关键词：
AGROBACTERIUM-MEDIATED TRANSFORMATION; BETA-VULGARIS L; DNA; TUMEFACIENS; EFFICIENCY; GROWTH; CELLS;
D O I：
10.1134/S000368381001014X
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
The parameters of transformation using Agrobacterium tumefaciens EHA 105 for 5 domestic sorts and lines of sugar beet (Beta vulgaris L. var. saccharifera (Alef) Krass) were optimized. The system of transgenic tissue selection based on resistance to phosphinothricin, allowing to avoid the appearing of chimeric shoots among initial transformants was developed. The transgenic plants of sugar beet sorts Ramonskaya single seed 47, L'govskaya single seed 52 and RMS 73, and LBO 17 and LBO 19 lines expressing the gene of phosphinothricin acetyl transferase bar have been obtained. The resistance of these sorts and lines to the effect of phosphinothricin in vitro has been shown.
引用
收藏
页码：80 / 86
页数：7
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