A low concentration of azithromycin inhibits the mRNA expression of N-acyl homoserine lactone synthesis enzymes, upstream of lasI or rhlI, in Pseudomonas aeruginosa

被引：41

作者：

Kai, Toshihiro ^{[2
]}

Tateda, Kazuhiro ^{[1
]}

Kimura, Soichiro ^{[1
]}

Ishii, Yoshikazu ^{[1
]}

Ito, Hideaki ^{[2
]}

Yoshida, Hiroaki ^{[2
]}

Kimura, Toru ^{[2
]}

Yamaguchi, Keizo ^{[1
]}

机构：

[1] Toho Univ, Sch Med, Dept Microbiol & Infect Dis, Ohta Ku, Tokyo 1438590, Japan

[2] Dainippon Sumitomo Pharma Co Ltd, Konohana Ku, Osaka 5540022, Japan

来源：

PULMONARY PHARMACOLOGY & THERAPEUTICS | 2009年 / 22卷 / 06期

关键词：

Macrolide; Quorum-sensing; DNA microarray; Pseudomonas aeruginosa; DIFFUSE PANBRONCHIOLITIS; MACROLIDE ANTIBIOTICS; VIRULENCE FACTORS; ERYTHROMYCIN; MECHANISM; THERAPY;

D O I：

10.1016/j.pupt.2009.04.004

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

Low-dose macrolides are effective therapy in patients with chronic lung infections, but the mechanisms of action are unclear. In this study, we performed DNA microarray analysis of Pseudomonas aeruginosa after treatment with a low concentration of azithromycin. We found that a sub-MIC of azithromycin didn't change mRNA expression of quorum-sensing related genes (lasI, lasR, rhlI, rhlR, vft, rsaL), but lowered expression of most N-aryl homoserine lactone (AHL) synthesis enzymes upstream of last and rhlI. We propose that small down regulation of these enzymes cumulatively resulted in a larger decrease of AHL production and inhibition of quorum-sensing in P. aeruginosa. (c) 2009 Elsevier Ltd. All rights reserved.

引用

页码：483 / 486

页数：4

共 10 条

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