SPIKES: Identification of physicochemical properties of spike proteins across diverse host species of SARS-CoV-2

被引：1

作者：

Sathipati, Srinivasulu Yerukala ^{[1
]}

Tsai, Ming-Ju ^{[2
,3
,4
]}

Carter, Tonia

Shukla, Sanjay K. ^{[1
]}

Ho, Shinn-Yin ^{[5
,6
,7
,8
]}

机构：

[1] Marshfield Clin Res Inst, Ctr Precis Med Res, Marshfield, WI 54449 USA

[2] Hinda & Arthur Marcus Inst Aging Res Hebrew Sr Lif, Boston, MA USA

[3] Beth Israel Deaconess Med Ctr, Dept Med, Boston, MA USA

[4] Harvard Med Sch, Boston, MA USA

[5] Natl Yang Ming Chiao Tung Univ, Inst Bioinformat & Syst Biol, Hsinchu, Taiwan

[6] Natl Yang Ming Chiao Tung Univ, Dept Biol Sci & Technol, Hsinchu, Taiwan

[7] Natl Yang Ming Chiao Tung Univ, Ctr intelligent Drug Syst & Smart Biodevices IDS2B, Hsinchu, Taiwan

[8] Kaohsiung Med Univ, Coll Hlth Sci, Kaohsiung, Taiwan

来源：

STAR PROTOCOLS | 2022年 / 3卷 / 03期

关键词：

SECONDARY STRUCTURE;

D O I：

10.1016/j.xpro.2022.101460

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We describe a protocol to identify physicochemical properties using amino acid sequences of spike (S) proteins of SARS-CoV-2. We present an S protein prediction technique named SPIKES, incorporating an inheritable bi-objective combinatorial genetic algorithm to determine the host species specificity. This protocol addresses the S protein amino acid sequence data collection, preprocessing, methodology, and analysis. For complete details on the use and execution of this protocol, please refer to Yerukala Sathipati et al. (2022).

引用

页数：15

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