Single-cell RNA sequencing of human kidney

被引：145

作者：

Liao, Jinling ^{[1
,2
,3
]}

Yu, Zhenyuan ^{[1
,2
,3
,4
,5
]}

Chen, Yang ^{[1
,2
,3
,4
,5
]}

Bao, Mengying ^{[1
,2
,3
]}

Zou, Chunlin ^{[6
,7
,8
]}

Zhang, Haiying ^{[1
,2
,3
]}

Liu, Deyun ^{[4
,5
]}

Li, Tianyu ^{[4
,5
]}

Zhang, Qingyun ^{[1
,2
,3
,4
,9
]}

Li, Jiaping ^{[10
,11
,12
]}

Cheng, Jiwen ^{[1
,2
,3
,4
,5
]}

Mo, Zengnan ^{[1
,2
,3
,4
,5
]}

机构：

[1] Guangxi Med Univ, Ctr Genom & Personalized Med, Nanning 530021, Guangxi, Peoples R China

[2] Guangxi Collaborat Innovat Ctr Genom & Personaliz, Nanning 530021, Guangxi, Peoples R China

[3] Guangxi Key Lab Coll & Univ, Guangxi Key Lab Genom & Personalized Med, Nanning 530021, Guangxi, Peoples R China

[4] Guangxi Med Univ, Affiliated Hosp 1, Inst Urol & Nephrol, Nanning 530021, Guangxi, Peoples R China

[5] Guangxi Med Univ, Dept Urol, Affiliated Hosp 1, Nanning 530021, Guangxi, Peoples R China

[6] Guangxi Med Univ, Minist Educ, Key Lab Longev & Aging Related Dis, Nanning 530021, Guangxi, Peoples R China

[7] Guangxi Med Univ, Ctr Translat Med, Nanning 530021, Guangxi, Peoples R China

[8] Guangxi Med Univ, Sch Preclin Med, Nanning 530021, Guangxi, Peoples R China

[9] Guangxi Med Univ, Dept Urol, Affiliated Tumour Hosp, Nanning 530021, Guangxi, Peoples R China

[10] Guangxi Med Univ, Dept Cardiol, Affiliated Hosp 1, Nanning 530021, Guangxi, Peoples R China

[11] Guangxi Key Lab Precis Med Cardiocerebrovasc Dis, Nanning 530021, Guangxi, Peoples R China

[12] Guangxi Clin Res Ctr Cardiocerebrovasc Dis, Nanning 530021, Guangxi, Peoples R China

来源：

SCIENTIFIC DATA | 2020年 / 7卷 / 01期

基金：

国家重点研发计划; 中国国家自然科学基金;

关键词：

GENE-EXPRESSION; LOCALIZATION;

D O I：

10.1038/s41597-019-0351-8

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

A comprehensive cellular anatomy of normal human kidney is crucial to address the cellular origins of renal disease and renal cancer. Some kidney diseases may be cell type-specific, especially renal tubular cells. To investigate the classification and transcriptomic information of the human kidney, we rapidly obtained a single-cell suspension of the kidney and conducted single-cell RNA sequencing (scRNA-seq). Here, we present the scRNA-seq data of 23,366 high-quality cells from the kidneys of three human donors. In this dataset, we show 10 clusters of normal human renal cells. Due to the high quality of single-cell transcriptomic information, proximal tubule (PT) cells were classified into three subtypes and collecting ducts cells into two subtypes. Collectively, our data provide a reliable reference for studies on renal cell biology and kidney disease.

引用

页数：9

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