Evaluation of different methods for assessing the reproductive mode of weeping lovegrass plants, Eragrostis curvula (Schrad.) Nees

被引:17
|
作者
Meier, Mauro [1 ,2 ]
Zappacosta, Diego [1 ,2 ]
Pablo Selva, Juan [1 ,2 ]
Pessino, Silvina [3 ]
Echenique, Viviana [1 ,2 ]
机构
[1] Univ Nacl Sur, CERZOS CONICET, CCT Bahia Blanca, Lab Biotecnol Vegetal, RA-8000 Bahia Blanca, Buenos Aires, Argentina
[2] Univ Nacl Sur, Dept Agron, RA-8000 Bahia Blanca, Buenos Aires, Argentina
[3] Univ Nacl Rosario, Fac Ciencias Agrarias, Mol Biol Lab, Provincia De Santa Fe, Argentina
关键词
TETRAPLOID POPULATIONS; PASPALUM-NOTATUM; RAPD ANALYSIS; CELL-WALLS; APOMIXIS; MEGASPOROGENESIS; EXPRESSION; CALLOSE; POLYMORPHISMS; BAHIAGRASS;
D O I
10.1071/BT10267
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Weeping lovegrass is a forage grass cultivated in semiarid regions of the world that reproduces mainly by apomixis (diplospory), a process that involves the formation of asexual seeds and bypasses the processes of meiosis and fertilisation. The aim of this work was to evaluate and compare different techniques (cytoembryology, callose deposition, flow cytometry and progeny tests) to determine the reproductive mode of weeping lovegrass. Typical sexual and apomictic processes were clearly differentiated using cytoembryology, and different callose deposition patterns were observed in sexual and apomictic genotypes. Previous studies indicated that presence of callose on the cell wall of the megaspore mother cell is associated only with sexual processes. Nevertheless, our results also found callose deposition in apomictic genotypes, although clearly different from the pattern found in sexual processes, allowing discrimination between sexual and apomictic plants. Flow cytometry seed screening using individual seeds did not differentiate between sexual and apomictic plants as the embryo : endosperm DNA content ratio was similar in sexual and apomictic plants. Progeny tests using molecular markers showed uniform patterns in offspring from apomictic plants and variable patterns among the progeny of sexual plants. The results obtained from cytological studies and progeny tests were similar, indicating that both methods provide useful tools for determination of reproductive mode. However, the callose test with aniline blue was faster and easier to use than other techniques.
引用
收藏
页码:253 / 261
页数:9
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