Morphological observation of nuclear sub-localization of human angiogenin in HUVECs

被引：0
|
作者：
Wu, Jie [1 ]
Zhang, Hong-Bin [1 ]
Wang, Jie [1 ]
Yang, Tai-Cheng [1 ]
Xian, Jiang [1 ]
Yang, Chuan-Hong [1 ]
Lai, Huang-Wen [1 ]
机构：
[1] Guangzhou Liuhuaqiao Hosp, Dept Med Res, Guangzhou 510010, Guangdong, Peoples R China
来源：
BIOTECHNOLOGY LETTERS | 2008年 / 30卷 / 06期
关键词：
angiogenin; expression; localization;
D O I：
10.1007/s10529-008-9651-3
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Angiogenin, a potent angiogenic factor, was cloned and expressed by Escherichia coli and then purified with gel filtration chromatography. Approximately 90% pure angiogenin was obtained to generate a monoclonal antibody. Using western immunoblotting and ELISA, we confirmed that monoclonal antibody C46 secreted from hybridoma cells stably and specifically binds to angiogenin. The fused protein angiogenin-EGF was then expressed in HUVECs, and the subcellular localization of the recombinant protein was determined by confocal microscopy and TEM assay. Recombinant angiogenin was found to mainly concentrate in the pars granulosa of the nucleus, where the protein accumulates to form ribonucleoprotein particles.
引用
收藏
页码：989 / 993
页数：5
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