Streptomyces pharmamarensis sp nov isolated from a marine sediment

A Gram-stain-positive actinobacterium, strain PM267(T), was isolated from a marine sediment sample in the Mediterranean Sea. The novel strain produced extensively branched substrate and aerial hyphae that carried spiral spore chains. Substrate and aerial mycelia were cream-white and white, respectively. Diffusible pigments were not observed. 16S rRNA gene sequence analysis revealed that strain PM267(T) belonged to the genus Streptomyces and shared a gene sequence similarity of 97.1 % with Streptomyces artemisiae YIM 63135(T) and Streptomyces armeniacus JCM 3070(T). Values <97% were obtained with other sequences representing members of the genus Streptomyces. The cell wall peptidoglycan contained LL-diaminopimelic acid. MK-9(H-8) was the major menaquinone. The phospholipid pattern included phosphatidylethanolamine as diagnostic lipid (type II). Major fatty acids found were iso- and anteiso- fatty acids. The G + C content of the DNA was 71.2 mol%. The strain was halotolerant and was able to grow in the presence of 90/s (w/v) NaCl (with an optimum of 2%). On the basis of these results and additional physiological data obtained in the present study, strain PM267(T) represents a novel species within the genus Streptomyces for which the name Streptomyces pharmamarensis sp. nov. is proposed (type strain PM267(T) =CECT 7841(T) =DSM 42032(T)).