DNA binding and cleavage properties of the Ce(III) complex of a diaza-crown ether

被引：6

作者：

Li, Fangzhen ^{[1
]}

Feng, Famei ^{[2
]}

Wu, Jiaoyi ^{[3
]}

Xie, Jiaqing ^{[1
]}

Li, Shuo ^{[1
]}

机构：

[1] Chongqing Univ Technol, Coll Chem & Chem Engn, Chongqing 400054, Peoples R China

[2] Sichuan Univ Sci & Engn, Coll Chem & Pharmaceut Engn, Zigong 643000, Sichuan, Peoples R China

[3] Peking Univ, Coll Chem & Mol Engn, Beijing 100871, Peoples R China

来源：

PROGRESS IN REACTION KINETICS AND MECHANISM | 2016年 / 41卷 / 01期

关键词：

aza-crown ether; cerium complex; DNA binding; DNA cleavage; enzyme mimic; MACROCYCLIC LANTHANIDE COMPLEXES; ARTIFICIAL NUCLEASES; CYTOTOXIC ACTIVITY; PHOSPHATE-ESTERS; HYDROLYSIS; DINUCLEAR; BIS(P-NITROPHENYL)PHOSPHATE; COFACTORS; LA(III); DESIGN;

D O I：

10.3184/146867816X14490554565537

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

The hydrolytic cleavage of pUC19 DNA as promoted by the trivalent cerium complex (CeL) of diaza-crown ether ligand L (1,4,10,13-tetraoxa-7,16-diazacyclooctadecane) was examined in detail. The interaction of CeL with calf thymus DNA was investigated by UV-Vis spectroscopy. Furthermore, studies on the effects of pH, reaction time, and the concentration of CeL on cleavage of pUC19 DNA were carried out by gel electrophoresis. The results indicate that CeL can bind to DNA electrostatically with a binding constant of 2.18x10(4) M-1; CeL can promote the cleavage of plasmid pUC19 DNA from supercoiled to the nicked form under the appropriate conditions, and the optimal pH value is 7.54. The lack of effect of radical scavengers indicates that the cleavage involves hydrolytic cleavage.

引用

页码：39 / 47

页数：9

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