A novel method for detection of apoptosis

被引:4
|
作者
Zagariya, Alexander M. [1 ]
机构
[1] Univ Illinois, Ctr Canc, Chicago, IL 60616 USA
关键词
Meconium; Lungs; Serine; Cysteine; Proteases; Apoptosis; II INDUCES APOPTOSIS; RECEPTOR-MEDIATED APOPTOSIS; ANGIOTENSIN-II; TYPE-2; RECEPTOR; CELLS; DIFFERENTIATION;
D O I
10.1016/j.yexcr.2012.02.001
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
There are two different Angiotensin II (ANG II) peptides in nature: Human type (ANG II) and Bovine type (ANG II*). These eight amino acid peptides differ only at position 5 where Valine is replaced by Isoleucine in the Bovine type. They are present in all species studied so far. These amino acids are different by only one atom of carbon. This difference is so small, that it will allow any of ANG II, Bovine or Human antibodies to interact with all species and create a universal method for apoptosis detection. ANG II concentrations are found at substantially higher levels in apoptotic, compared to non-apoptotic, tissues. ANG II accumulation can lead to DNA damage, mutations, carcinogenesis and cell death. We demonstrate that Bovine antiserum can be used for universal detection of apoptosis. In 2010, the worldwide market for apoptosis detection reached the $20 billion mark and significantly increases each year. Most commercially available methods are related to Annexin V and TUNNEL Our new method based on ANG II is more widely known to physicians and scientists compared to previously used methods. Our approach offers a novel alternative for assessing apoptosis activity with enhanced sensitivity, at a lower cost and ease of use. (C) 2012 Elsevier Inc. All rights reserved.
引用
收藏
页码:861 / 866
页数:6
相关论文
共 50 条
  • [1] A novel method for quantitative analysis of apoptosis
    Peng, LM
    Liu, JJ
    LABORATORY INVESTIGATION, 1997, 77 (06) : 547 - 555
  • [2] Apoptosis detection by annexin V binding: a novel method for the quantitation of cell-mediated cytotoxicity
    Shounan, Y
    Feng, XM
    O'Connell, PJ
    JOURNAL OF IMMUNOLOGICAL METHODS, 1998, 217 (1-2) : 61 - 70
  • [3] A novel assay for detection of amyloid induced apoptosis
    Paulsson, J. F.
    Schultz, S. W.
    Kohler, M.
    Leibiger, L.
    Berggren, P.-O.
    Westermark, G. T.
    XIth International Symposium on Amyloidosis, 2008, : 27 - 29
  • [4] Detection of apoptosis: A review of conventional and novel techniques
    Martinez, Michelle M.
    Reif, Randall D.
    Pappas, Dimitri
    ANALYTICAL METHODS, 2010, 2 (08) : 996 - 1004
  • [5] A novel assay for detection of amyloid induced apoptosis
    Paulsson, J. F.
    Westermark, G. T.
    AMYLOID-JOURNAL OF PROTEIN FOLDING DISORDERS, 2006, 13 : 52 - 52
  • [6] A NOVEL METHOD FOR THE DETECTION OF NITRATES
    GUPTA, HL
    PANDE, PN
    AMETA, SA
    JOURNAL OF CHEMICAL EDUCATION, 1980, 57 (04) : 319 - 319
  • [7] A novel intrusion detection method
    Jiang, SY
    Li, QH
    Wang, H
    NETWORK AND PARALLEL COMPUTING, PROCEEDINGS, 2004, 3222 : 459 - 462
  • [8] A Novel method for Target Detection
    Li, Xiangxiang
    Zhu, Songhao
    Chen, Lingling
    PROCEEDINGS OF THE 2015 INTERNATIONAL SYMPOSIUM ON COMPUTERS & INFORMATICS, 2015, 13 : 750 - 755
  • [9] A novel method for insulin detection
    Slachtova, Lenka
    Zemanova, Jitka
    Sacha, Pavel
    Fabre, Benjamin
    Zakova, Lenka
    Jiracek, Jiri
    Konvalinka, Jan
    FASEB JOURNAL, 2019, 33
  • [10] A novel contour detection method
    Wu, Rongteng
    OPTOELECTRONIC IMAGING AND MULTIMEDIA TECHNOLOGY II, 2012, 8558