Detoxification of arsenic by phytochelatins in plants

被引:408
|
作者
Schmöger, MEV
Oven, M
Grill, E
机构
[1] Tech Univ Munich, Lehrstuhl Bot, Biologikum Weihenstephan, D-85350 Freising Weihenstephan, Germany
[2] Univ Munich, Lehrstuhl Pharmazeut Biol, D-80333 Munich, Germany
关键词
D O I
10.1104/pp.122.3.793
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
As is a ubiquitous element present in the atmosphere as well as in the aquatic and terrestrial environments. Arsenite and arsenate are the major forms of As intoxication, and these anions are readily taken up by plants. Both anions efficiently induce the biosynthesis of phytochelatins (PCs) ([gamma-glutamate-cysteine](n)-glycine) in vivo and in vitro. The rapid induction of the metal-binding PCs has been observed in cell suspension cultures of Rauvolfia serpentina, in seedlings of Arabidopsis, and in enzyme preparations of Silene vulgaris upon challenge to arsenicals. The rate of PC formation in enzyme preparations was lower compared with Cd-induced biosynthesis, but was accompanied by a prolonged induction phase that resulted finally in higher peptide levels. An approximately 3:1 ratio of the sulfhydryl groups from PCs to As is compatible with reported As-glutathione complexes. The identity of the As-induced PCs and of reconstituted metal-peptide complexes has unequivocally been demonstrated by electrospray ionization mass spectroscopy. Gel filtration experiments and inhibitor studies also indicate a complexation and detoxification of As by the induced PCs.
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收藏
页码:793 / 801
页数:9
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