Electric Cell-Substrate Impedance Sensing (ECIS) as a Platform for Evaluating Barrier-Function Susceptibility and Damage from Pulmonary Atelectrauma

被引:0
|
作者
Yamaguchi, Eiichiro [1 ]
Yao, Joshua [1 ]
Aymond, Allison [1 ]
Chrisey, Douglas B. [2 ]
Nieman, Gary F. [3 ]
Bates, Jason H. T. [4 ]
Gaver, Donald P. [1 ]
机构
[1] Tulane Univ, Dept Biomed Engn, New Orleans, LA 70118 USA
[2] Tulane Univ, Dept Phys & Engn Phys, New Orleans, LA 70118 USA
[3] SUNY Upstate Med Univ, Dept Surg, Syracuse, NY 13210 USA
[4] Univ Vermont, Dept Med, Burlington, VT 05405 USA
来源
BIOSENSORS-BASEL | 2022年 / 12卷 / 06期
基金
美国国家卫生研究院;
关键词
ECIS technology; ARDS; VILI; atelectrauma; barrier function; pulmonary disease; lung; mechanical ventilation; ENDOTHELIAL-CELLS; MECHANICAL-STRESS; SURFACE-TENSION; MODEL;
D O I
10.3390/bios12060390
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
Biophysical insults that either reduce barrier function (COVID-19, smoke inhalation, aspiration, and inflammation) or increase mechanical stress (surfactant dysfunction) make the lung more susceptible to atelectrauma. We investigate the susceptibility and time-dependent disruption of barrier function associated with pulmonary atelectrauma of epithelial cells that occurs in acute respiratory distress syndrome (ARDS) and ventilator-induced lung injury (VILI). This in vitro study was performed using Electric Cell-substrate Impedance Sensing (ECIS) as a noninvasive evaluating technique for repetitive stress stimulus/response on monolayers of the human lung epithelial cell line NCI-H441. Atelectrauma was mimicked through recruitment/derecruitment (RD) of a semi-infinite air bubble to the fluid-occluded micro-channel. We show that a confluent monolayer with a high level of barrier function is nearly impervious to atelectrauma for hundreds of RD events. Nevertheless, barrier function is eventually diminished, and after a critical number of RD insults, the monolayer disintegrates exponentially. Confluent layers with lower initial barrier function are less resilient. These results indicate that the first line of defense from atelectrauma resides with intercellular binding. After disruption, the epithelial layer community protection is diminished and atelectrauma ensues. ECIS may provide a platform for identifying damaging stimuli, ventilation scenarios, or pharmaceuticals that can reduce susceptibility or enhance barrier-function recovery.
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页数:18
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