A biochemical fluorometric method for assessing the oxidative properties of HDL

被引：64
|
作者：
Kelesidis, Theodoros [1 ]
Currier, Judith S. [1 ]
Huynh, Diana [1 ]
Meriwether, David [2 ]
Charles-Schoeman, Christina [1 ]
Reddy, Srinivasa T. [1 ,2 ,3 ]
Fogelman, Alan M. [1 ]
Navab, Mohamad [1 ]
Yang, Otto O. [1 ,4 ]
机构：
[1] Univ Calif Los Angeles, David Geffen Sch Med, Dept Med, Los Angeles, CA 90095 USA
[2] Univ Calif Los Angeles, David Geffen Sch Med, Dept Obstet & Gynecol, Los Angeles, CA 90095 USA
[3] Univ Calif Los Angeles, David Geffen Sch Med, Dept Mol & Med Pharmacol, Los Angeles, CA 90095 USA
[4] Univ Calif Los Angeles, David Geffen Sch Med, Dept Microbiol Immunol & Mol Genet, Los Angeles, CA 90095 USA
来源：
JOURNAL OF LIPID RESEARCH | 2011年 / 52卷 / 12期
基金：
美国国家卫生研究院;
关键词：
dysfunctional high density lipoprotein; oxidation; dihydrorhodamine; 123; HDL function; HIGH-DENSITY-LIPOPROTEIN; PEROXYNITRITE-MEDIATED OXIDATION; APOLIPOPROTEIN-A-I; ANTIINFLAMMATORY PROPERTIES; INFLAMMATORY PROPERTIES; METHIONINE SULFOXIDE; LIPID HYDROPEROXIDES; CHOLESTEROL LEVELS; INHIBITS; PLASMA;
D O I：
10.1194/jlr.D018937
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Most current assays of HDL functional properties are cell-based. We have developed a fluorometric biochemical assay based on the oxidation of dihydrorhodamine 123 (DHR) by HDL. This cell-free assay assesses the intrinsic ability of HDL to be oxidized by measuring increasing fluorescence due to DHR oxidation over time. The assay distinguishes the oxidative potential of HDL taken from different persons, and the results are reproducible. Direct comparison of this measurement correlated well with results obtained using a validated cell-based assay (r(2) = 0.62, P < 0.001). The assay can be scaled from a 96-well format to a 384-well format and, therefore, is suitable for high-throughput implementation. This new fluorometric method offers an inexpensive, accurate, and rapid means for determining the oxidative properties of HDL that is applicable to large-scale clinical studies.-Kelesidis, T., J. S. Currier, D. Huynh, D. Meriwether, C. Charles-Schoeman, S. T. Reddy, A. M. Fogelman, M. Navab, and O. O. Yang. A biochemical fluorometric method for assessing the oxidative properties of HDL. J. Lipid Res. 2011. 52: 2341-2351.
引用
收藏
页码：2341 / 2351
页数：11
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