Isolation, characterization, and chromosomal mapping of a novel cDNA clone encoding human selenium binding protein

被引:1
|
作者
Chang, PWG
Tsui, SKW
Liew, CC
Lee, CY
Waye, MMY
Fung, KP
机构
[1] CHINESE UNIV HONG KONG,DEPT BIOCHEM,SHATIN,NT,HONG KONG
[2] UNIV TORONTO,DEPT CLIN BIOCHEM,TORONTO,ON,CANADA
关键词
selenium binding protein; human heart cDNA; fluorescent in situ hybridization; chromosome; 1;
D O I
10.1002/(SICI)1097-4644(199702)64:2<217::AID-JCB5>3.0.CO;2-#
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We have isolated the full-length human 56 kDa selenium binding protein (hSPS56) cDNA clone, which is the human homolog of mouse 56 kDa selenium binding protein. The cDNA is 1,668 bp long and has an open reading frame encoding 472 amino acids. The calculated molecular weight is 52.25 kDa and the estimated isoelectric point is 6.13. Using Northern blot hybridization, we found that this 56 kDa selenium binding protein is expressed in mouse heart with an intermediate level between those found in liver/lung/kidney and intestine. We have also successfully expressed hSP56 in Escherichia coli using the expression vector-pAED4. The hSP56 gene is located at human chromosome 1q21-22. (C) 1997 Wiley-Liss, Inc.
引用
收藏
页码:217 / 224
页数:8
相关论文
共 50 条
  • [1] ISOLATION AND SEQUENCE OF A CDNA CLONE ENCODING HUMAN CHROMOSOMAL PROTEIN HMG-1
    WEN, L
    HUANG, JK
    JOHNSON, BH
    REECK, GR
    [J]. FASEB JOURNAL, 1988, 2 (05): : A1016 - A1016
  • [2] ISOLATION AND CHARACTERIZATION OF A MAIZE CDNA CLONE ENCODING THE TATA BOX BINDING-PROTEIN, TFIID
    HAASS, MM
    FEIX, G
    [J]. BIOLOGICAL CHEMISTRY HOPPE-SEYLER, 1991, 372 (09): : 667 - 667
  • [3] ISOLATION AND CHARACTERIZATION OF A CDNA CLONE ENCODING THE TATA BOX-BINDING PROTEIN (TFIID) FROM WHEAT
    KAWATA, T
    MINAMI, M
    TAMURA, T
    SUMITA, K
    IWABUCHI, M
    [J]. PLANT MOLECULAR BIOLOGY, 1992, 19 (05) : 867 - 872
  • [4] ISOLATION OF A CDNA CLONE ENCODING A NOVEL MEMBRANE-PROTEIN EXPRESSED IN LYMPHOCYTES
    SHIMIZU, Y
    OGAWA, H
    OKA, Y
    MIZUNO, R
    SAKODA, S
    KISHIMOTO, T
    SUGIYAMA, H
    [J]. FEBS LETTERS, 1994, 355 (01) : 30 - 34
  • [5] ISOLATION AND CHARACTERIZATION OF A CDNA CLONE ENCODING A PLEURODELES LECTIN
    TIFFOCHE, C
    CHESNEL, A
    JEGO, P
    LEPENNEC, JP
    [J]. EUROPEAN JOURNAL OF BIOCHEMISTRY, 1993, 213 (03): : 901 - 907
  • [6] Isolation and characterization of a cDNA clone encoding a putative white spruce glycine-rich RNA binding protein
    Richard, S
    Drevet, C
    Jouanin, L
    Séguin, A
    [J]. GENE, 1999, 240 (02) : 379 - 388
  • [7] ISOLATION AND CHARACTERIZATION OF CDNA CLONE ENCODING A FACTOR-BINDING TO THE EPO GENE PROMOTER
    GUPTA, M
    MUNGAI, P
    GOLDWASSER, E
    [J]. BLOOD, 1993, 82 (10) : A316 - A316
  • [8] Isolation and expression of a cDNA clone encoding human kynureninase
    AlberatiGiani, D
    Buchli, R
    Malherbe, P
    Broger, C
    Lang, G
    Kohler, C
    Lahm, HW
    Cesura, AM
    [J]. EUROPEAN JOURNAL OF BIOCHEMISTRY, 1996, 239 (02): : 460 - 468
  • [9] ISOLATION OF THE CDNA CLONE FOR A HUMAN FOLATE BINDING-PROTEIN (FBP)
    SADASIVAN, E
    ROTHENBERG, SP
    [J]. FEDERATION PROCEEDINGS, 1987, 46 (03) : 1004 - 1004
  • [10] CHARACTERIZATION AND CHROMOSOMAL MAPPING OF A GENOMIC CLONE ENCODING HUMAN ALANINE - GLYOXYLATE AMINOTRANSFERASE
    PURDUE, PE
    LUMB, MJ
    FOX, M
    GRIFFO, G
    HAMONBENAIS, C
    POVEY, S
    DANPURE, CJ
    [J]. GENOMICS, 1991, 10 (01) : 34 - 42