Development and validation of a microarray for the confirmation and typing of norovirus RT-PCR products

被引：9

作者：

Mattison, Kirsten ^{[1
,2
]}

Corneau, Nathalie ^{[1
]}

Berg, Ingvild ^{[3
]}

Bosch, Albert ^{[4
]}

Duizer, Erwin ^{[5
]}

Gutierrez-Aguirre, Ion ^{[6
]}

L'Homme, Yvan ^{[7
]}

Lucero, Yalda ^{[8
]}

Luo, Zhiyao ^{[7
]}

Martyres, Alexander ^{[2
]}

Myrmel, Mette ^{[3
]}

O'Ryan, Miguel ^{[8
]}

Pagotto, Franco ^{[1
,2
]}

Sano, Daisuke ^{[4
]}

Svraka, Sanela ^{[5
]}

Urzua, Ulises ^{[8
]}

Bidawid, Sabah ^{[1
]}

机构：

[1] Hlth Canada, Bur Microbial Hazards, Ottawa, ON K1A 0K9, Canada

[2] Univ Ottawa, Dept Biochem Microbiol & Immunol, Ottawa, ON, Canada

[3] Norwegian Sch Vet Sci, Dept Food Safety & Infect Biol, Oslo, Norway

[4] Univ Barcelona, Dept Microbiol, Barcelona, Spain

[5] Natl Inst Publ Hlth & Environm, Dept Virol, NL-3720 BA Bilthoven, Netherlands

[6] Natl Inst Biol, Dept Biotechnol & Syst Biol, Ljubljana, Slovenia

[7] Canadian Food Inspect Agcy, St Hyacinthe Lab, St Hyacinthe, PQ, Canada

[8] Univ Chile, Fac Med, Santiago 7, Chile

来源：

JOURNAL OF VIROLOGICAL METHODS | 2011年 / 173卷 / 02期

关键词：

Norovirus; Microarray; Genotyping; NORWALK-LIKE VIRUSES; REVERSE TRANSCRIPTION-PCR; ROUND-STRUCTURED VIRUSES; VIRAL GASTROENTERITIS; DNA MICROARRAY; HYBRIDIZATION; OUTBREAKS; ASSAY; EPIDEMIOLOGY; OPTIMIZATION;

D O I：

10.1016/j.jviromet.2011.02.011

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Noroviruses are implicated in many worldwide institutional, food and waterborne outbreaks each year. Genetic typing of isolates is valuable for monitoring outbreak spread as well as variation in circulating strains. Microarrays have the potential to provide rapid genotype information for norovirus samples. The NoroChip v3.0 provides an oligonucleotide hybridization platform to screen for over 600 potential interactions in each experiment. The NoroChip v3.0 was developed at Health Canada and validated in seven international partner laboratories. Each laboratory validated the NoroChip v3.0 using norovirus amplicons routinely characterized in their testing protocols. Fragments from the capsid region (region C) and a 2.4 kb amplicon spanning polymerase and capsid sequences (region AD) were validated in six of the partner laboratories and provided correct genogroup typing information (GI or GII) when hybridized to the NoroChip v3.0. Results indicate that the current limiting factor for implementing the NoroChip v3.0 as a strain typing tool is the difficulty obtaining a long, specific amplicon from all circulating norovirus strains. Data obtained with the longer region AD amplicon provided the best discrimination between norovirus strains. Crown Copyright (C) 2011 Published by Elsevier B.V. All rights reserved.

引用

页码：233 / 250

页数：18

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