Light-regulated gene expression in Bacteria: Fundamentals, advances, and perspectives

被引:14
|
作者
Ohlendorf, Robert [1 ]
Moeglich, Andreas [2 ,3 ,4 ]
机构
[1] MIT, Dept Biol Engn, 77 Massachusetts Ave, Cambridge, MA 02139 USA
[2] Univ Bayreuth, Dept Biochem, Bayreuth, Germany
[3] Univ Bayreuth, Bayreuth Ctr Biochem & Mol Biol, Bayreuth, Germany
[4] Univ Bayreuth, North Bavarian NMR Ctr, Bayreuth, Germany
关键词
biotechnology; gene expression; optogenetics; sensory photoreceptor; signal transduction; synthetic biology; INFRARED FLUORESCENT PROTEINS; ENGINEERING ESCHERICHIA-COLI; PSEUDOMONAS-AERUGINOSA; SPATIOTEMPORAL CONTROL; ADENYLYL-CYCLASE; BINDING DOMAIN; LOV2; DOMAIN; DNA-BINDING; TRANSCRIPTIONAL ACTIVATION; CYANOBACTERIOCHROME CCAS;
D O I
10.3389/fbioe.2022.1029403
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Numerous photoreceptors and genetic circuits emerged over the past two decades and now enable the light-dependent i.e., optogenetic, regulation of gene expression in bacteria. Prompted by light cues in the near-ultraviolet to near-infrared region of the electromagnetic spectrum, gene expression can be up- or downregulated stringently, reversibly, non-invasively, and with precision in space and time. Here, we survey the underlying principles, available options, and prominent examples of optogenetically regulated gene expression in bacteria. While transcription initiation and elongation remain most important for optogenetic intervention, other processes e.g., translation and downstream events, were also rendered light-dependent. The optogenetic control of bacterial expression predominantly employs but three fundamental strategies: light-sensitive two-component systems, oligomerization reactions, and second-messenger signaling. Certain optogenetic circuits moved beyond the proof-of-principle and stood the test of practice. They enable unprecedented applications in three major areas. First, light-dependent expression underpins novel concepts and strategies for enhanced yields in microbial production processes. Second, light-responsive bacteria can be optogenetically stimulated while residing within the bodies of animals, thus prompting the secretion of compounds that grant health benefits to the animal host. Third, optogenetics allows the generation of precisely structured, novel biomaterials. These applications jointly testify to the maturity of the optogenetic approach and serve as blueprints bound to inspire and template innovative use cases of light-regulated gene expression in bacteria. Researchers pursuing these lines can choose from an ever-growing, versatile, and efficient toolkit of optogenetic circuits.
引用
收藏
页数:39
相关论文
共 50 条
  • [1] Analysis of light-regulated gene expression
    Dijkwel, PP
    Rook, F
    Smeekens, SCM
    [J]. PHOTOSYNTHESIS: MOLECULAR BIOLOGY OF ENERGY CAPTURE, 1998, 297 : 182 - 191
  • [2] LIGHT-REGULATED GENE-EXPRESSION
    SIMPSON, J
    HERRERAESTRELLA, L
    [J]. CRITICAL REVIEWS IN PLANT SCIENCES, 1990, 9 (01) : 95 - 109
  • [3] Dynamic Histone Modifications in Light-Regulated Gene Expression
    Hofmann, Nancy R.
    [J]. PLANT CELL, 2009, 21 (12): : 3717 - 3717
  • [4] CIRCADIAN RHYTHMICITY IN THE EXPRESSION OF A NOVEL LIGHT-REGULATED RICE GENE
    REIMMANN, C
    DUDLER, R
    [J]. PLANT MOLECULAR BIOLOGY, 1993, 22 (01) : 165 - 170
  • [5] Light-regulated gene repositioning in Arabidopsis
    Feng, Chun-Miao
    Qiu, Yongjian
    Van Buskirk, Elise K.
    Yang, Emily J.
    Chen, Meng
    [J]. NATURE COMMUNICATIONS, 2014, 5 : 3027
  • [6] Light-regulated gene repositioning in Arabidopsis
    Chun-Miao Feng
    Yongjian Qiu
    Elise K. Van Buskirk
    Emily J. Yang
    Meng Chen
    [J]. Nature Communications, 5
  • [7] PLATE-BASED ASSAYS FOR LIGHT-REGULATED GENE EXPRESSION SYSTEMS
    Tabor, Jeffrey J.
    [J]. METHODS IN ENZYMOLOGY, VOL 497: SYNTHETIC BIOLOGY, METHODS FOR PART/DEVICE CHARACTERIZATION AND CHASSIS ENGINEERING, PT A, 2011, 497 : 373 - 391
  • [8] Visible Light-Regulated Gene Expression and Neurite Outgrowth of Nerve Cells
    Ling, Qing-Dong
    Ho, Ling-Yi
    Ko, Yi-An
    Chang, Yung
    Higuchi, Akon
    [J]. JOURNAL OF CHEMICAL ENGINEERING OF JAPAN, 2011, 44 (03) : 171 - 178
  • [9] LIGHT-REGULATED GENE-EXPRESSION DURING MAIZE LEAF DEVELOPMENT
    NELSON, T
    HARPSTER, MH
    MAYFIELD, SP
    TAYLOR, WC
    [J]. JOURNAL OF CELL BIOLOGY, 1984, 98 (02): : 558 - 564
  • [10] A clock- and light-regulated gene that links the circadian oscillator to LHCB gene expression
    Xu, Y
    Johnson, CH
    [J]. PLANT CELL, 2001, 13 (06): : 1411 - 1425