The three-dimensional imaging of microspheres in confocal and conventional polarization microscopes

被引：0
|
作者：
Yang, LS [1 ]
Taylor, CM [1 ]
Rakovich, Y [1 ]
McCabe, EM [1 ]
机构：
[1] Univ Dublin Trinity Coll, Dept Phys, Dublin 2, Ireland
来源：
THREE-DIMENSIONAL AND MULTIDIMENSIONAL MICROSCOPY: IMAGE ACQUISITION AND PROCESSING X | 2003年 / 4964卷
关键词：
three-dimensional microscopy; polarization; microsphere;
D O I：
10.1117/12.477978
中图分类号：
TH742 [显微镜];
学科分类号：
摘要：
Images of the microspheres are studied in three-dimensions by using the confocal and conventional scanning polarization microscopes. It is found that the polarization of the detected signals is mainly parallel to the initial polarization which is due to the high extinction coefficient of the confocal system. Arc pairs are observed at the edge of the microspheres with the conventional polarization microscope with a crossed analyzer. Theoretical analysis are given by using the vector field theory and the image formations of the two systems.
引用
收藏
页码：66 / 72
页数：7
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