Structural characterization of Botryosphaeran:: a (1→3;1→6)-β-D-glucan produced by the ascomyceteous fungus, Botryosphaeria sp.

被引：97
|
作者：
Barbosa, AM
Steluti, RM
Dekker, RFH
Cardoso, MS
da Silva, MLC [1 ]
机构：
[1] Univ Estadual Paulista, Fac Ciencias & Tecnol, Dept Quim Fis & Biol, BR-19060900 Presidente Prudente, SP, Brazil
[2] Univ Oeste Paulista, Dept Bioquim, BR-19050900 Presidente Prudente, SP, Brazil
[3] Univ Estadul Londrina, Dept Bioquim, BR-86051990 Londrina, PR, Brazil
来源：
CARBOHYDRATE RESEARCH | 2003年 / 338卷 / 16期
基金：
巴西圣保罗研究基金会;
关键词：
exopolysaccharide; (1 -> 3; 1 -> 6)-beta-glucan; Botryosphaeran; Botyrosphaeria sp; ascomyceteous fungus;
D O I：
10.1016/S0008-6215(03)00240-4
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The exopolysaccharide, Botryosphaeran, produced by the ligninolytic, ascomycetcous fungus Botryosphaeria sp., was isolated from the extracellular fluid by precipitation with ethanol, and purified by gel permeation chromatography to yield a carbohydrate-rich fraction (96%) composed mainly of glucose (98%). Infra-red and C-13 NMR spectroscopy showed that all the glucosidic linkages were in the beta-configuration. Data from methylation analysis and Smith degradation indicated that Botryosphaeran was a (1 --> 3)-beta-(D)-glucan with approx 22% side branching at C-6. The products obtained from partial acid hydrolysis demonstrated that the side branches consisted of single (1 --> 6)-beta-linked glucosyl, and (1 --> 6)-beta-linked gentiobiosyl residues. [GRAPHICS] (C) 2003 Elsevier Ltd. All rights reserved.
引用
收藏
页码：1691 / 1698
页数：8
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