Two Ways to Achieve the Same Goal-Two Validated Quantitative NMR Strategies for a Low-Abundance Natural Product in Standardized Extracts: The Case of Hepatodamianol in Turnera diffusa

被引：0

作者：

Parra-Naranjo, Aida ^{[1
]}

Delgado-Montemayor, Cecilia ^{[1
]}

Salazar-Aranda, Ricardo ^{[1
]}

Castro-Rios, Rocio ^{[1
]}

Saucedo, Alma L. ^{[1
,2
]}

Waksman-Minsky, Noemi ^{[1
]}

机构：

[1] Univ Autonoma Nuevo Leon, Fac Med, Dept Quim Analit, Monterrey 64460, Mexico

[2] Consejo Nacl Ciencia & Technol, Ciudad De Mexico 03940, Mexico

来源：

MOLECULES | 2022年 / 27卷 / 19期

关键词：

qNMR; method validation; nuclear magnetic resonance; natural products; damiana; QUALITY-CONTROL; METABOLITE QUANTIFICATION; QNMR METHOD; H-1-NMR; PURITY; ACCURATE; PULCON; MARKER;

D O I：

10.3390/molecules27196593

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The quantification of low-abundance secondary metabolites in plant extracts is an analytical problem that can be addressed by different analytical platforms, the most common being those based on chromatographic methods coupled to a high-sensitivity detection system. However, in recent years nuclear magnetic resonance (NMR) has become an analytical tool of primary choice for this type of problem because of its reliability, inherent simplicity in sample preparation, reduced analysis time, and low solvent consumption. The versatility of strategies based on quantitative NMR (qNMR), such as internal and external standards and electronic references, among others, and the need to develop validated analytical methods make it essential to compare procedures that must rigorously satisfy the analytical well-established acceptance criteria for method validation. In this work, two qNMR methods were developed for the quantification of hepatodamianol, a bioactive component of T. diffusa. The first method was based on a conventional external standard calibration, and the second one was based on the pulse length-based concentration determination (PULCON) method using the ERETIC2 module as a quantitation tool available in TopSpin software. The results show that both procedures allow the content of the analyte of interest in a complex matrix to be determined in a satisfactory way, under strict analytical criteria. In addition, ERETIC2 offers additional advantages such as a reduction in experimental time, reagent consumption, and waste generated.

引用

页数：16