Antimicrobial Properties of Microparticles Based on Carmellose Cross-Linked by Cu2+ Ions

被引:3
|
作者
Kejdusova, Martina [1 ]
Vyslouzil, Jakub [1 ]
Kubova, Katerina [1 ]
Celer, Vladimir [2 ]
Krasna, Magdalena [2 ]
Pechova, Alena [3 ]
Vyskocilova, Vjra [3 ]
Kost'al, Vratislav [4 ]
机构
[1] Univ Vet & Pharmaceut Sci Brno, Fac Pharm, Dept Pharmaceut, Brno 61242, Czech Republic
[2] Univ Vet & Pharmaceut Sci Brno, Fac Vet Med, Dept Infect Dis & Microbiol, Brno 61242, Czech Republic
[3] Univ Vet & Pharmaceut Sci Brno, Fac Vet Hyg & Ecol, Dept Biochem & Biophys, Brno 61242, Czech Republic
[4] Tescan, Brno 62300, Czech Republic
关键词
CARBOXYMETHYL CELLULOSE; COPPER NANOPARTICLES; IN-VITRO; DRUG-DELIVERY; MICROSPHERES; PH; COMPLEXES; HYDROGELS; CHITOSAN; PROTEIN;
D O I
10.1155/2015/790720
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Carmellose (CMC) is frequently used due to its high biocompatibility, biodegradability, and low immunogenicity for development of site-specific or controlled release drug delivery systems. In this experimental work, CMC dispersions in two different concentrations (1% and 2%) cross-linked by copper (II) ions (0.5, 1, 1.5, or 2.0 M CuCl2) were used to prepare microspheres with antimicrobial activity against Escherichia coli and Candida albicans, both frequently occurring pathogens which cause vaginal infections. The microparticles were prepared by an ionotropic gelation technique which offers the unique possibility to entrap divalent copper ions in a CMC structure and thus ensure their antibacterial activity. Prepared CMC microspheres exhibited sufficient sphericity. Both equivalent diameter and copper content were influenced by CMC concentration, and the molarity of copper (II) solution affected only the copper content results. Selected samples exhibited stable but pH-responsive behaviour in environments which corresponded with natural (pH 4.5) and inflamed (pH 6.0) vaginal conditions. All the tested samples exhibited proven substantial antimicrobial activity against both Gram-negative bacteria Escherichia coli and yeast Candida albicans. Unexpectedly, a crucial parameter for microsphere antimicrobial activity was not found in the copper content but in the swelling capacity of the microparticles and in the degree of CMC surface shrinking.
引用
收藏
页数:9
相关论文
共 50 条
  • [1] Crystalline Structure of Chitosan Microgranules Cross-Linked with Cu2+ and Ag+ Ions
    Modrzejewska, Zofia
    Binias, Dorota
    Wojtasz-Pajak, Anna
    Dorabialska, Malgorzata
    Zarzycki, Roman
    [J]. CRYSTAL GROWTH & DESIGN, 2008, 8 (12) : 4372 - 4377
  • [2] Synthesis and Adsorption Properties of Cu2+ Imprinted Cross-linked Chitosan Microspheres
    Fu, Ming-Lian
    Huang, Qiu-Yun
    Ye, Yu-Juan
    Chen, Zhang-Xu
    [J]. Jingxi Huagong/Fine Chemicals, 2019, 36 (06): : 1228 - 1234
  • [3] Cu2+ Coordination of Covalently Cross-linked β-Amyloid Dimers
    Simon C. Drew
    W. Mei Kok
    Craig A. Hutton
    Kevin J. Barnham
    [J]. Applied Magnetic Resonance, 2013, 44 : 927 - 939
  • [4] Cu2+ Coordination of Covalently Cross-linked β-Amyloid Dimers
    Drew, Simon C.
    Kok, W. Mei
    Hutton, Craig A.
    Barnham, Kevin J.
    [J]. APPLIED MAGNETIC RESONANCE, 2013, 44 (08) : 927 - 939
  • [5] Erratum to: Cu2+ Coordination of Covalently Cross-linked β-Amyloid Dimers
    Simon C. Drew
    W. Mei Kok
    Craig A. Hutton
    Kevin J. Barnham
    [J]. Applied Magnetic Resonance, 2014, 45 : 621 - 621
  • [6] Investigation of Cross-Linked Chitosan-Based Membranes as Potential Adsorbents for the Removal of Cu2+ Ions from Aqueous Solutions
    Vlachou, Irene
    Bokias, Georgios
    [J]. MATERIALS, 2023, 16 (05)
  • [7] Synthesis and characterization of guar gum-based cross-linked graft copolymers for the adsorption of Cu2+ ions from aqueous solution
    Sharma, Rajeev Kr
    Kumar, Rajesh
    [J]. TRENDS IN CARBOHYDRATE RESEARCH, 2019, 11 (04) : 15 - 27
  • [8] Adsorption of Cd2+ and Cu2+ ions from aqueous solutions by a cross-linked polysulfonate-carboxylate resin
    Haladu, Shamsuddeen A.
    Al-Hamouz, Othman Charles S.
    Ali, Shaikh A.
    [J]. ARABIAN JOURNAL OF CHEMISTRY, 2019, 12 (08) : 2597 - 2607
  • [9] Shape memory behaviour of polyvinyl alcohol aerogels cross-linked with Fe3+ & Cu2+ metallic ions
    Mohsenian, Zahra
    Kokabi, Mehrdad
    Alamdarnejad, Ghazaleh
    [J]. SMART MATERIALS AND STRUCTURES, 2023, 32 (08)
  • [10] Compostable properties of antimicrobial bioplastics based on cinnamaldehyde cross-linked gliadins
    Balaguer, Mari Pau
    Villanova, Joan
    Cesar, Guy
    Gavara, Rafael
    Hernandez-Munoz, Pilar
    [J]. CHEMICAL ENGINEERING JOURNAL, 2015, 262 : 447 - 455