Radical Scavenging Activity of Spring Mountain Herbs in the Shikoku Mountain Area and Identification of Antiradical Constituents by Simple HPLC Detection and LC-MS Methods

被引:4
|
作者
Masuda, Toshiya [1 ]
Inouchi, Tomoko [1 ]
Fujimoto, Aya [1 ]
Shingai, Yoshimi [1 ]
Inai, Miyuki [1 ]
Nakamura, Mitsuhiro [1 ]
Imai, Shoji [1 ]
机构
[1] Univ Tokushima, Grad Sch Integrated Arts & Sci, Tokushima 7708502, Japan
关键词
mountain herb; radical scavenging activity; Polystichym ovato-paleaceum (Tsuyanashiinode); Sambucus racemosa subsp sieboldiana (Niwatoko); ANTIOXIDANT PROPERTIES; PHENOLIC CONTENT; PLANT; EXTRACTS; L;
D O I
10.1271/bbb.110837
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The functionality of spring mountain herbs, which were collected in the Kajigamori mountain area of Shikoku area in Japan, was investigated in the course of our studies for utilizing local plant resources. The radical scavenging activity of the extracts from seventeen herbs was measured. Among these herbs, two extracts from Polystichym ovato-paleaceum (Japanese name: Tsuyanashiinode) and Sambucus racemosa subsp. sieboldiana (Japanese name: Niwatoko) showed potent DPPH radical scavenging activity. The material evidence for the potent activity of the extracts was studied by a combination of our developed method for detecting antiradical compounds, LC-MS/MS, and enzymatic hydrolysis.
引用
收藏
页码:705 / 711
页数:7
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