In vitro oocyte maturation in Polycystic Ovarian Syndrome patients

Prevalence of Polycystic Ovarian Syndrome(PCOS) in Iran is more than 6%. Therefore we encounter with many PCOS patients. In Vitro Maturation (IVM) of oocytes as an attractive method in ART is considered. Studies show that changes in culture conditions should be administered to make IVM protocol more successful. For this purpose in this study we have set up the beneficial cultures for IVM procedure. Fourteen PCOS patients received FSH, 75 IU or 150 IU per day for 3 days initiating on day 3 of menstruation. Oocyte retrieval was performed transvaginally using an ultrasound-guided 17-gauge single lumen needle and filtered through a 70 micron gauge filter. Viable oocytes were put to maturation in TCM-199 supplemented with 10% Patient serum, recombinant FSH, pyruvate, penicillin, streptomycin sulphate and human chorionic gonadotropin.. Oocytes were then inseminated by ICSI. The results indicated that 43.4% of oocytes matured to metaphase II. After 48 hours 47.5 % of M II oocytes fertilized by ICSI and cleaved to 2- and 4-cell stage. No pregnancy observed in PCOS patients. The oocytes maturation rate (43.4%) and embryo formation (47.5 %) from immature oocytes obtained in our IVM and ICSI culture system indicate that the present system may be nearly good, even though the number of patients were too small to draw significant conclusions.