Analyses in transgenic tobacco of the promoter from a Brassica napus gene highly expressed in the stigma

被引：5
|
作者：
Robert, LS
Lévesque-Lemay, M
Gerster, JL
Hong, HP
Keller, W
机构：
[1] Eastern Cereal & Oilseed Res Ctr, Ottawa, ON K1A 0C6, Canada
[2] Natl Res Council Canada, Inst Plant Biotechnol, Saskatoon, SK S7N 0W9, Canada
来源：
PLANT CELL REPORTS | 1999年 / 18卷 / 05期
关键词：
Brassica; beta-glucuronidase; promoter; stigma; tobacco;
D O I：
10.1007/s002990050586
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
A genomic clone, Pis G363, containing the Brassica napus stigma-expressed gene Pis 63-2 was isolated and sequenced. The coding region of Pis G363 does not possess introns and shows 82% identity to the nucleotide sequence of a gene from Arabidopsis BAC clone T01B08. A 2-kb promoter fragment from Pis G363 was fused to the coding sequence of the marker enzyme beta-glucuronidase (GUS) and introduced into tobacco via Agrobacterium-mediated transformation. The promoter fragment directed expression of the GUS gene in the stigma of transgenic tobacco. Some transformants also showed relatively low GUS activity in the pollen.
引用
收藏
页码：357 / 362
页数：6
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