Nanocapsulation of curcumin and its protective effects against oxidative stress and carcinoma HepG2, MCF7 cells

Curcumin, the highly active compound has restrictedin themedical and nutritional applications due to its poor bioavailability and solubility. UsingBall milling technique for grinding curcuma powder at 30, 90 min and capsulated the natural extracts in micro and nano forms. Chemical components were compared between control (0 min) powder with 90 min grinded time. Comparing the non-capsulated (CM),Microencapsulated (CMm) and nanoencapsulated (CMn) as biological and chemical agents. UsingHPLC-MS, it was found three main components; curcumin, curcumin glucuronid and curcumin sulfate at different ratios.Moreover, SEM microscope showedaverage particle size more than 450-550 mu m for micro CMm 90 capsules, while TEM microscope showedspherical micellesranged between 7 -42 nm for nano CMn 90 capsules.Microencapsulated (CMm) and nanoencapsulated (CMn) were investigated as antioxidant and anticancer agents.Samples (66.6 mu g/ml) were showed obvious DPPH scavenging activity for micro-then nanoencapsuled, and that was the same in slightly trend for Reducing power compared with non-encapsulated and ascorbic acid. Against human hepato cancer HepG2 cells, nanoencapsules were more active than micro capsules followed by non-capsuled samples in a bright selective anticancer activity. Against human breast cancer MCF7, the anticancer potential has higher IC50(mu g/ml) but in the same activity trend.