Transient expression of UreB of Helicobacter pylori in spinach (Spinacia oleracea)

Helicobacter pylori that exists in the human gastric mucosa has been known the main cause of chronic gastritis, peptic ulcers and stomach cancer. Vaccination against H. pylori has been considered as the most effective way to prevent the bacterial infection. UreB has been identified as the most effective antigen in all of the H. pylori strains. Because of potential advantages of plant expression system for Production of Recombinant Vaccine, in this study, the UreB gene was transferred into Spinach Leaves (Spinacia oleracea) for transient expression using three separate instructions mediated using Agrobacterium. Several signal peptides were considered in gene construct to increase protein expression and purification. The efficiency of different instructions, confirmation of transformation and protein expression was evaluated using RT-PCR, Real time-PCR, Dot blotting, ELISA assay and SDS-PAGE. Also, it was compared with the UreB expression in E. coli BL21 strain induced by Isopropyl B-D-1-Thiogalactopyranoside. The highest protein expression level was approximately 68% of the expression level of this protein in E. coli. This is the first report of UreB expression in Spinach.