Mangrovitalea sediminis gen. nov., sp nov., a member of the family Alteromonadaceae isolated from mangrove sediment

A Gram-stain-negative, facultative anaerobic and oligotrophic, rod-shaped, and motile with single polar flagellum bacterial strain, designed M11-4 (T) was isolated from mangrove sediment in Yunxiao Mangrove National Nature Reserve, China. Growth was observed at temperatures from 10 to 40 degrees C (optimum 30 degrees C), at salinities from 0.5 to 6% (optimum 2-3 %), and at pH from 5 to 8 (optimum 6). Phylogenetic analysis based on 16S rRNA gene sequence revealed that strain M11-4 (T) shared highest sequence similarity with the genus Marinobacter (92.5-95.0 %) and represented a distinct phylogenetic lineage in the family Alteromonadaceae. The G+ C content of the genomic DNA was 58.2 mol%. The dominant fatty acids were C-16 : 0, C-16 : 0 Nalcohol, summed feature 9 (comprising iso-C-17 : 1 omega 9c and/or C-16 : 0 10-methyl) and summed feature 3 (comprising C-16 : 1 omega 7c and/or C-16 : 1 omega 6c). The predominant respiratory quinone was ubiquinone-9 and the major polar lipids were diphosphatidylglycerol; phosphatidylethanolamine; phosphatidylglycerol and an unidentified aminophospholipid. According to its morphology, physiology, fatty acid composition and 16S rRNA gene sequence analysis, the strain M11-4 T should be assigned as a novel species of a novel genus for which the name Mangrovitalea sediminis gen. nov., sp. nov. is proposed. The type strain of Mangrovitalea sediminis is M11-4 (T) (= MCCC 1K03312 (T) = JCM 32104 (T)).