The Arabidopsis Nitrate Transporter NRT2.4 Plays a Double Role in Roots and Shoots of Nitrogen-Straved Plants

被引：295

作者：

Kiba, Takatoshi ^{[2
]}

Feria-Bourrellier, Ana-Belen ^{[1
]}

Lafouge, Florence ^{[1
]}

Lezhneva, Lina ^{[1
]}

Boutet-Mercey, Stephanie ^{[1
]}

Orsel, Mathilde ^{[1
]}

Brehaut, Virginie ^{[1
]}

Miller, Anthony ^{[3
]}

Daniel-Vedele, Francoise ^{[1
]}

Sakakibara, Hitoshi ^{[2
]}

Krapp, Anne ^{[1
]}

机构：

[1] Inst Natl Rech Agron AgroParisTech, Inst Jean Pierre Bourgin, Unite Mixte Rech 1318, F-78026 Versailles, France

[2] RIKEN Plant Sci Ctr, Yokohama, Kanagawa 2300045, Japan

[3] John Innes Ctr, Dept Dis & Stress Biol, Norwich NR4 7UH, Norfolk, England

来源：

PLANT CELL | 2012年 / 24卷 / 01期

基金：

英国生物技术与生命科学研究理事会;

关键词：

GENE-EXPRESSION; RICINUS-COMMUNIS; UPTAKE SYSTEM; PHLOEM-SAP; NO3-UPTAKE; AFFINITY; THALIANA; CHL1; REMOBILIZATION; METABOLISM;

D O I：

10.1105/tpc.111.092221

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Plants have evolved a variety of mechanisms to adapt to N starvation. NITRATE TRANSPORTER2.4 (NRT2.4) is one of seven NRT2 family genes in Arabidopsis thaliana, and NRT2.4 expression is induced under N starvation. Green fluorescent protein and beta-glucuronidase reporter analyses revealed that NRT2.4 is a plasma membrane transporter expressed in the epidermis of lateral roots and in or close to the shoot phloem. The spatiotemporal expression pattern of NRT2.4 in roots is complementary with that of the major high-affinity nitrate transporter NTR2.1. Functional analysis in Xenopus laevis oocytes and in planta showed that NRT2.4 is a nitrate transporter functioning in the high-affinity range. In N-starved nrt2.4 mutants, nitrate uptake under low external supply and nitrate content in shoot phloem exudates was decreased. In the absence of NRT2.1 and NRT2.2, loss of function of NRT2.4 (triple mutants) has an impact on biomass production under low nitrate supply. Together, our results demonstrate that NRT2.4 is a nitrate transporter that has a role in both roots and shoots under N starvation.

引用

页码：245 / 258

页数：14

共 5 条

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[J]. FRONTIERS IN PLANT SCIENCE, 2024, 15
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