Molecular typing of Staphylococcus aureus based on PCR-RFLP of coa gene and RAPD analysis

被引：7
|
作者：
Karakulska, J. [1 ]
Pobucewicz, A. [1 ]
Nawrotek, P. [1 ]
Muszynska, M. [2 ]
Furowicz, A. J. [1 ]
Czernomysy-Furowicz, D. [1 ]
机构：
[1] W Pomeranian Univ Technol Szczecin, Fac Biotechnol & Anim Sci, Dept Immunol Microbiol & Physiol Chem, PL-71466 Szczecin, Poland
[2] W Pomeranian Univ Technol Szczecin, Fac Biotechnol & Anim Sci, Lab Mol Cytogenet, Dept Ruminants Sci, PL-71466 Szczecin, Poland
来源：
POLISH JOURNAL OF VETERINARY SCIENCES | 2011年 / 14卷 / 02期
关键词：
S; aureus; mastitis; nuc gene; gap gene; coa gene; PCR-RFLP; RAPD; BOVINE MASTITIS; COAGULASE GENE; POLYMORPHISM; MILK; COWS;
D O I：
10.2478/v10181-011-0044-5
中图分类号：
S85 [动物医学（兽医学）];
学科分类号：
0906 ;
摘要：
The aim of this study was molecular identification of S. aureus strains isolated from mastitic milk samples and establishing the genetic relationship between strains isolated from cows belonging to the same herd. In all 43 isolated strains the gap gene (930 bp) was amplified, which enabled their affiliation to the Staphylococcus genus to be established. PCR-RFLP with AluI endonuclease of the gap gene as well as nuc (450 bp) and coa (1130 bp) gene amplification allowed precise S. aureus species identification. One hundred percent of the genetic relationship between strains was established via RAPD-PCR and coa-typing.
引用
收藏
页码：285 / 286
页数：2
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