Development of EST-SSR in foxtail millet (Setaria italica)

被引:93
|
作者
Jia, Xiao-Ping
Shi, Yun-Su
Song, Yan-Cun
Wang, Guo-Ying
Wang, Tian-Yu [1 ]
Li, Yu
机构
[1] China Agr Univ, State Key Lab Agribiotechnol, Beijing 100094, Peoples R China
[2] Chinese Acad Agr Sci, Inst Crop Sci, NFCRI, Beijing 100081, Peoples R China
关键词
EST-SSR; foxtail millet; polymorphism; Setaria italica; transferability;
D O I
10.1007/s10722-006-9139-8
中图分类号
S3 [农学(农艺学)];
学科分类号
0901 ;
摘要
The development of EST-SSR in foxtail millet (Setaria italica) for polymorphism and transferability study was reported here. From 1213 EST sequences, 30 SSRs were obtained and primers were designed for 26 SSRs. Among them, four pairs of SSR primers amplified polymorphic products in 12 foxtail millet cultivars and one accession of Setaria viridis, a wild relative of foxtail millet, with 10 alleles detected for the four loci and 2.5 alleles per locus. In addition, ten SSR markers could be transferred to other nine Gramineae species. The putative functions of 11 ESTs containing polymorphic and transferable SSRs were also identified.
引用
收藏
页码:233 / 236
页数:4
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