Detection of Listeria monocytogenes by polymerase chain reaction oriented to inlB gene

被引：1

作者：

Pangallo, D ^{[1
]}

Kaclíková, E ^{[1
]}

Kuchta, T ^{[1
]}

Drahovská, H ^{[1
]}

机构：

[1] Food Res Inst, Dept Microbiol & Chem, SK-82475 Bratislava 26, Slovakia

来源：

MICROBIOLOGICA | 2001年 / 24卷 / 04期

关键词：

Listeria monocytogenes; PCR; internalin;

D O I：

暂无

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Primers were designed for the detection of Listeria monocytogenes by the polymerase chain reaction oriented to specific sequences of the inlB gene encoding an internalin. At optimized reaction conditions, 100% sensitivity (on a panel of 33 strains of L. monocytogenes) and 100% specificity (on panels of 15 strains of other Listeria spp. and 41 other bacteria), were determined for the inlB-L/R primers. The detection limit of PCR with these primers was 10(4) cfu/ml and was not affected by up to 10(R)cfu/ml of L. innoctta.

引用

页码：333 / 339

页数：7

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