Flavobacterium dasani sp. nov., a psychrotolerant bacterium isolated from Arctic soil

A novel yellow-colored, Gram-stain-negative, aerobic, non-motile, catalase- and oxidase-positive, and rod-shaped psychrotolerant bacterium, designated strain PLR-18-3(T), was isolated from Arctic soil and was subjected to polyphasic taxonomic study. Cells were able to grow at 0-30 degrees C, pH 6.0-10.5, and 0-3.0% (w/v) NaCl concentration. Based on the 16S rRNA gene sequence analysis, this Arctic strain belonged to the genus Flavobacterium, with the closest neighbor being Flavobacterium noncentrifugens R-HLS-17(T) (96.2% sequence similarity). The strain contained MK-6 as a sole respiratory quinone, phosphatidylethanolamine as the major polar lipid, and summed feature 3 (C(16:1)7c and/or C(16:1)6c), iso-C-15:0, iso-C-15:0 G, iso-C-17:0 3-OH, iso-C-15:0 3-OH, anteiso-C-15:0, and summed feature 9 (iso-C(17:1)9c and/or C(16:0)10-methyl) as the predominant fatty acids. The DNA G+C content was 37.9mol%. On the basis of polyphasic data, strain PLR-18-3(T) represents a novel species of the genus Flavobacterium, for which the name Flavobacterium dasani sp. nov. is proposed. The type strain is PLR-18-3(T) (=KEMB 9005-713(T)=KACC 19627(T)=NBRC 113347(T)).