EFFECTS OF DIFFERENT LAND USES ON COMMUNITY STRUCTURE OF SOIL ARBUSCULAR MYCORRHIZAL FUNGI IN SANJIANG WETLANDS

The community of Arbuscular mycorrhizal fungi (AMF) was compared between the soils obtained from wetlands, rice agricultural land and artificial forest. In order to explore the influence of human activities on the soil ecology of Sanjiang wetland in China, total microbial DNA was isolated from the soil and AMF-specific amplicons were sequenced by high-throughput sequencing on an Illumina MiSeq platform. The observed variation in soil AMF community structures for the three land-use type soils was presented here for 30 OTUs with high relative abundance and for 14 AMF genera that could be recognized. The total OTU abundance and diversity was highest in wetland soil, lower in agricultural land and lowest in forest soil. Members of Claroideoglomus, Funneliformis, Gigaspora and Diversispora were commonly detected genera in all the three samples. RDA analysis showed that these dominant AMF genera correlated negatively with the acidity of the soil, with the exception of Diversispora, which correlated negatively with nutrient (N, P) content. This indicated that long-term farming and forestry practices have a significant impact on soil AMF community structures in wetland ecosystems.