GnRH receptor messenger ribonucleic acid expression in bovine ovary

The present study was undertaken to investigate the mRNA expression for gonadotropin-releasing hormone (GnRH) receptor in bovine ovary. Granulosa cells from small (< 4 mM); medium (5-8 mM) and large follicles (> 8 mM) and tissues from corpora lutea (CL) of different stages: Stage I (days 1-4), Stage II (days 5-10), Stage III (days 11-17), and Stage IV (days 18-21, days after ovulation) were harvested from bovine ovaries collected at a local abattoir. The mRNA isolated from representative samples was subjected to reverse transcription-polymerase chain reaction (RT-PCR) using gene sequence specific primers. The resultant PCR amplified gonadotropin-releasing hormone receptor (GnRH-R) cDNA products were identified and confirmed through Southern blot hybridization and nucleotide sequence analysis, respectively. The results showed the presence of GnRH-R mRNA transcripts in both follicles and CL.