We prove ratio asymptotic for sequences of multiple orthogonal polynomials with respect to a Nikishin system of measures N(sigma(l),..., sigma(m)) such that for each k, sigma(k) has constant sign on its support consisting on an interval Delta(k), on which vertical bar sigma(')(k) vertical bar > 0 almost everywhere, and a set without accumulation points in R\ Delta(k). 2008 Published by Elsevier Inc.