Kaiso, a new protein of the BTB/POZ family, specifically binds to methylated DNA sequences

被引：7
|
作者：
Prokhortchouk, AV [1 ]
Aitkhozhina, DS
Sablina, AA
Ruzov, AS
Prokhortchouk, EB
机构：
[1] Russian Acad Sci, Inst Gene Biol, Moscow 117334, Russia
[2] Russian Acad Med Sci, Blokhin Inst Carcinogenesis, Moscow 115478, Russia
来源：
RUSSIAN JOURNAL OF GENETICS | 2001年 / 37卷 / 06期
关键词：
D O I：
10.1023/A:1016660921580
中图分类号：
Q3 [遗传学];
学科分类号：
071007 ; 090102 ;
摘要：
A protein specifically binding to a symmetrically methylated DNA fragment of the first intron of the mts1 gene was studied. The protein was purified by gel filtration and affinity chromatography. Mass spectrometry showed that the protein is Kaiso, a new member of the BTB/POZ family. To study the association with methylated DNA sequences in vivo, the location of Kaiso in NIH 3T3 cells was analyzed. Immunofluorescent staining with polyclonal antibodies against Kaiso showed that the protein is predominantly associated with the nucleoli. The causes of its distribution awaits further investigation. The zinc-finger domains of Kaiso were for the first time demonstrated to specifically recognize symmetrically methylated DNA sequences in vitro.
引用
收藏
页码：603 / 609
页数：7
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