Three-dimensional autofluorescence spectroscopy of rat skeletal muscle tissue under two-photon excitation

被引：28
|
作者：
Schilders, SP [1 ]
Gu, M [1 ]
机构：
[1] Victoria Univ Technol, Sch Commun & Informat, Optoelect Imaging Grp, Melbourne, Vic 8001, Australia
来源：
APPLIED OPTICS | 1999年 / 38卷 / 04期
关键词：
D O I：
10.1364/AO.38.000720
中图分类号：
O43 [光学];
学科分类号：
070207 ; 0803 ;
摘要：
We report on three-dimensional autofluorescence spectroscopy obtained from rat skeletal muscle tissue under two-photon excitation by an ultrashort pulsed-laser beam. It is demonstrated that two types of fluorophores within the skeletal muscle tissue can be simultaneously excited with the laser beam at a wavelength of 800 nm. The two fluorophores exhibited unique fluorescence spectral peaks at wavelengths of 450 and 550 nm. These spectroscopic signals can be used to form a three-dimensional image, giving the information about the biochemical makeup of the skeletal muscle tissue. (C) 1999 Optical Society of America OCIS codes: 170.6280, 170.6900, 170.6930.
引用
收藏
页码：720 / 723
页数：4
相关论文
共 50 条
  • [1] Single - and two-photon excitation autofluorescence Spectroscopy of diabetic rat artery
    Xu, Gaixia
    Qu, Junle
    Guo, Xuan
    Guo, Baoping
    Niu, Hanben
    [J]. PROCEEDINGS OF INTERNATIONAL SYMPOSIUM ON BIOPHOTONICS, NANOPHOTONICS AND METAMATERIALS, 2006, : 186 - +
  • [2] Two-photon excitation of fluorescence in three-dimensional microscopy
    Diaspro, A
    Robello, M
    [J]. EUROPEAN JOURNAL OF HISTOCHEMISTRY, 1999, 43 (03): : 169 - 178
  • [3] Two-photon autofluorescence spectroscopy of oral mucosa tissue
    Edward, Kert.
    Shilagard, Tuya
    Qiu, Suimin
    Vargas, Gracie
    [J]. MULTIPHOTON MICROSCOPY IN THE BIOMEDICAL SCIENCES XI, 2011, 7903
  • [4] Three-dimensional focal spots related to two-photon excitation
    Sun, HB
    Tanaka, T
    Kawata, S
    [J]. APPLIED PHYSICS LETTERS, 2002, 80 (20) : 3673 - 3675
  • [5] Autofluorescence of epithelial tissue: single-photon versus two-photon excitation
    Zheng, Wei
    Wu, Yicong
    Li, Dong
    Qu, Jianan Y.
    [J]. JOURNAL OF BIOMEDICAL OPTICS, 2008, 13 (05)
  • [6] Three-dimensional optical functional imaging of tissue with two-photon excitation laser scanning microscopy
    Masters, BR
    [J]. ANALYTICAL USE OF FLUORESCENT PROBES IN ONCOLOGY, 1996, 286 : 205 - 211
  • [7] Two-photon autofluorescence microscopy of multicolor excitation
    Li, Dong
    Zheng, Wei
    Qu, Jianan Y.
    [J]. OPTICS LETTERS, 2009, 34 (02) : 202 - 204
  • [8] Three-dimensional localisation of fluorescence resonance energy transfer in living cells under two-photon excitation
    Xu, MG
    Crimeen, B
    Ludford-Menting, MJ
    Gan, XS
    Russell, SM
    Gu, M
    [J]. SCANNING, 2001, 23 (01) : 9 - 13
  • [9] Two-photon excitation of fluorescence for three-dimensional optical imaging of biological structures
    Diaspro, A
    Robello, M
    [J]. JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY, 2000, 55 (01) : 1 - 8
  • [10] Three-Dimensional Microfabrication by Two-Photon Lithography
    Da Yang
    Shalin J. Jhaveri
    Christopher K. Ober
    [J]. MRS Bulletin, 2005, 30 : 976 - 982
12345