Establishment and application of autoradiograph assay of p38 MAPK activity

被引：0

作者：

Yan, WS ^{[1
]}

Jiang, Y ^{[1
]}

Huang, QB ^{[1
]}

Zhao, KS ^{[1
]}

机构：

[1] First Mil Med Univ, Dept Pathophysiol, Guangzhou 510515, Peoples R China

来源：

PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS | 2001年 / 28卷 / 03期

关键词：

p38 mitogen-activated protein kinase; autoradiograph assay of kinase activity; lipopolysaccharide; vascular endothelial cell;

D O I：

暂无

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Autoradiograph assay of p38 MAPK (mitogen-activated protein kinase) activity was established, and applied in measurement of p38 MAPK activity of vascular endothelial cell stimulated by lipopolysaccharide (LPS). Results showed that autoradiograph assay possessed specificity and sensitivity. Activity of p38 MAPK in endothelial cell treated by LPS was increased at 15 min, and reached maximum value during 30 similar to 60 min, then descended. It suggested that domestic laboratory completely had the ability to establish autoradiograph assay of kinase activity, and applied in study of signal transduction.

引用

页码：410 / 414

页数：5

共 5 条

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