Separation and Enrichment of Ginsenosides from Extracts of Root of Panax Notoginsen Burk. F. H. Chen. by Foam Floatation-Solid Phase Extraction

Foam floatation-solid phase extraction was applied to the separation and enrichment of ginsenosides from extracts of Panax notoginsen (Burk.) F. H. Chen. The selectivity and sensitivity for determining the ginsenosides were improved. The experimental conditions, including the flow rate of nitrogen gas, floatation time and pH value of sample solution, for foam floatation and elution conditions for SPE were examined and optimized. Average recoveries for protopanaxdiol (PPD) ginsenosides Rc, Rb(2), Rb(2), Rd, and Rb(1) were between 85.01% and 97.51%. The relative standard deviations (RSDs) were lower than 5.8% for the PPD ginsenosides. The FF-SPE was applied to both the separation and concentration for the PPD ginsenosides. The proposed method shows a potential in the separation and concentration of trace analytes in the solution samples.